Biological Characteristics of Biosurfactantproducing Petroleum-degrader Bacterium Bacillus BS-8  

产生物表面活性剂的石油降解菌Bacillus BS-8的生物学特性研究（英文）

作　　者：常慧萍[1] 邢文会[1] 夏铁骑[2] 付瑞敏[1] 陶令霞[2] 张红[1] 

机构地区：[1]河南教育学院生命科学系,河南郑州450046 [2]濮阳职业技术学院,河南濮阳457000

出　　处：《Agricultural Science & Technology》2016年第1期1-3,18,共4页农业科学与技术（英文版）

基　　金：Supported by Basic and Cutting-edge Technique Research of Henan Province(152300410092);Key Program in Science and Technology of Education Department of Henan Province(15A210020,15B180002,15B180016)~~

摘　　要：The growth characteristics of petroleum-degrader BS-8（Bacillus sp.） and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included： glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃.研究石油降解菌BS-8(Bacillus sp.)的生长特性及影响其产生物表面活性剂的因素。通过测定BS-8发酵液的OD600、表面张力、排油圈直径推测其表面活性剂的产生方式;考察了碳源、氮源、温度、pH、NaCl浓度对其产生物表面活性剂的影响。结果表明,菌株BS-8的表面活性剂产生方式为生长相关型,发酵液的表面张力随菌体数量的增加而降低,排油圈直径与发酵液中表面活性剂含量呈正相关;菌株BS-8高产表面活性剂的碳源、氮源分别为葡萄糖、酵母膏,最适培养温度为30℃,最适pH 7.0及最适NaCl浓度为2.0%。

关 键 词：BIOSURFACTANT Petroleum-degrader BS-8 Growth-correlated Biological characteristic 

分 类 号：Q939.9[生物学—微生物学]