阿里红多糖对卡氏肺孢子菌肺炎大鼠外周血TNF-α、sICAM-1和IL-8和脾脏T细胞亚群的影响  

The effect of Fomes Officinalis Polysaccharides on the contents of TNF- α、s ICAM- 1 、IL- 8 in peripheral serum and the Lymphocyte subsets of T cells in Splenic of rats with Pneumocystis carinii pneumonia

作　　者：陈永杰[1] 吕小川[2] 高剑[3] 伊丽孜拉.伊克拉木 赖星星[1] 孙艳[1] 刘云飞[1] 

机构地区：[1]新疆医科大学 [2]新疆医科大学第六附属医院呼吸消化科 [3]新疆医科大学基础医学院人体寄生虫学教研室

出　　处：《医学动物防制》2016年第2期179-182,186,共5页Journal of Medical Pest Control

基　　金：国家级大学生创新创业训练计划项目(201310760002);新疆医科大学大学生创新性实验计划项目(CX2013002);新疆医科大学博士(后)科研项目启动基金并新疆医科大学科研创新基金(XJ201222)

摘　　要：目的探索阿里红多糖对卡氏肺孢子菌肺炎大鼠外周血清中TNF-α、s ICAM和IL-8 3种细胞因子含量及大鼠脾脏T淋巴细胞亚型分布的影响。方法建立SD大鼠PCP动物模型并随机分为5组,设立PCP模型对照组及阿里红多糖(Fomes Officinalis Polysaccharides,FOP)低(14 mg/kg)、中(28 mg/kg)、高剂量(56 mg/kg)治疗组,以复方新诺明作为阳性药物对照组,并设立空白SD大鼠为正常对照。ELISA检测各实验组大鼠外周血清中TNF-α、s ICAM-1和IL-8 3种细胞因子含量,流式细胞术分别检测PCP模型组和高剂量FOP组大鼠脾脏T淋巴细胞中CD4+T和CD8+T细胞百分比。结果各剂量阿里红多糖均能显著下调PCP大鼠外周血清中TNF-α、s ICAM-1的表达,且下调作用随FOP剂量增加而加大,组间结果比较差异有统计学意义(TNF-α:F=3 567.47,P<0.01;s ICAM-1:F=5 845.48,P<0.01);低剂量FOP组大鼠外周血清中IL-8含量高于PCP模型组,中剂量FOP组大鼠外周血清中IL-8含量低于PCP模型组(F=6.971,P<0.01),高剂量FOP组大鼠外周血清中IL-8含量与PCP模型组相比显著下降,但与SMZ-TMP阳性药物对照组相比差异无统计学意义(F=0.003,P>0.05);高剂量FOP组大鼠脾脏T细胞亚群分布中CD4+T细胞百分比显著增高,与PCP模型组大鼠相比差异有统计学意义(F=903.316,P<0.01)。结论阿里红多糖对PCP的治疗机制主要与其对机体免疫功能的调节作用有关,可能通过上调CD4+T细胞百分比和下调机体外周血清中PCP发生相关炎性介质的表达有关。Objective To study the effect of Fomes Officinalis Polysaccharides on the contents of TNF- α、s ICAM- 1、IL- 8 in peripheral serum and the percentages of T cells in Splenic of rats with Pneumocystis carinii pneumonia. Methods PCP rat models were established and then divided the model into five groups randomly： PCP model control group,Low dose Fo P group（ 14 mg / kg）,Moderate dose Fo P group（ 28 mg / kg）,High dose Fo P group（ 56 mg / kg）,Cotrimoxazole control group. At the same time normal control group was established. The contents of TNF- α、s ICAM- 1、IL- 8 in peripheral serum of rats in each group were detected by ELISA,and the percentages of CD4＋T cells and CD8＋T in Splenic of rats in PCP model control group and High dose Fo P group were detected by flow cytometry（ FCM）. Results Each dose of FOP could significantly downregulated the expression of TNF- α and s ICAM- 1 in peripheral serum of rats with PCP（ TNF- α： F = 3 567. 47,P 〈0. 01; s ICAM- 1： F = 5 845. 48,P 〈0. 01）,and the downregulation increased with the increase of dose. The contents of IL- 8 in peripheral serum of rats in Low dose Fo P group were higher than the PCP model control group,weal lower in the Moderate dose Fo P group（ F =6. 971,P〈 0. 01）. There were no significant differences compared with the Cotrimoxazole control group（ F = 0. 003,P〈 0. 05）,the High dose Fo P could significantly downregulated the expression of IL- 8 in peripheral serum of rats with PCP,and also could increase the percentages of CD4＋T cells in Splenic of PCP rats（ F = 903. 316,P 〈0. 01）.

关 键 词：卡氏肺孢子虫肺炎 阿里红多糖 TNF-Α s ICAM-1 IL-8 T细胞亚群 

分 类 号：R382.3[医药卫生—医学寄生虫学]