对照提取物定性和单标定量相结合的白芷药材质量控制研究  

Quality control of Angelica dahurica by standardized reference extract qualification and single compound quantitative evaluation

作　　者：李德强[1] 段立广[2] 郑旭光[2] 戴荣源 张志清[1] 

机构地区：[1]河北医科大学第二医院药学部,石家庄050000 [2]河北医科大学第一医院药剂科,石家庄050000 [3]中国临床药理学杂志,北京100191

出　　处：《中国临床药理学杂志》2016年第4期333-336,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省中医药管理局项目基金资助项目(2014138)

摘　　要：目的评价用对照提取物定性和一测多评定量相结合的方法对白芷药材进行质量控制。方法用高效液相色谱法,色谱柱:ACE Ultra Core C_(18)(50 mm×2.1 mm,2.5μm),流动相:乙腈-水梯度洗脱,流速:0.5 mL·min^(-1),柱温:30℃,检测波长:310 nm,进样量:5μL。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性以及重现性。结果高效液相色谱法对白芷中的5种主要香豆素类成分,佛手柑内酯、氧化前胡素、欧前胡素、cinidilin和异欧前胡素进行了快速分离,线性关系良好(r≥0.999 4),回收率较高,均在96.4%~100.0%内。对照提取物能快速对白芷中的5种主要成分进行定性。单标定量方法测得成分含量与标准曲线法定量的结果一致。结论对照提取物定性和单标定量相结合的质量控制方法是简单、可行的,能有效地对白芷药材进行质量控制。Objective Quality control of Angelica dahurica by standardized reference extract qualification and single compound quantitative evaluation. Methods The samples were analyzed by HPLC after pressurized liquid extraction（ PLE）. The chromatographic conditions were as following： The column was ACE Ultra Core C_（18）（ 50 mm × 2. 1 mm,2. 5 μm）;the mobile phase consisted of acetonitrile- water using gradient elution;the flow rate was 1. 0 mL · min^（-1） and the column temperature was 30℃. The detection wavelength was set at 310 nm and the sample injection volume was 5 μL. The method was validated by investigating its specificity,linearities and limit of detection,precision,extraction recovery,stability and repeatability. Results Five components including bergapten,oxypeucedanin, imperatorin, cinidilin and isoimperatorin were determined by HPLC. The linearities of them were good（ r≥0. 999 4） in the concentration ranges of 0. 50- 10. 00,1. 00- 20. 00,1. 00- 20. 0,1. 00- 20. 00,1. 00- 20. 00 mL · L^（- 1）,respectively. The coumarins showed the overall recoveries ranging from 96. 4%- 100. 0%. The peaks of determined compounds were identified by standardized reference extract. The contents of coumarins in samples were respectively determined by individual calibration curve method and single compound quantitative evaluation. The results had a good consistence,but the contents of fivecompounds of Angelica dahurica from different areas had obvious differences. Conclusion The strategy is feasible and credible,which is easily and effectively adapted for the quality control of Angelica dahurica.

关 键 词：白芷 质量控制 对照提取物 单标定量 

分 类 号：R284.2[医药卫生—中药学] R932[医药卫生—中医学]