RP-HPLC法同时测定西洋参含片中4种成分  

Simultaneous determination of four components in Panax quinque folius L.Tablets by RP-HPLC

作　　者：闫玉梅[1] 赵梅梅[1] 王笑笑[1] 方佳成 廖莎[1] 王世祥[1] 郑晓晖[1,2] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]深圳清华大学研究院,深圳518057

出　　处：《西北药学杂志》2016年第1期5-8,共4页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81303185);陕西省重点科技创新团队计划(编号:2013KCT-24);高等学校博士学科点专项科研基金项目(编号:20136101120030)

摘　　要：目的建立同时测定西洋参含片中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1及拟人参皂苷F11含量的RP-HPLC方法。方法色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm);流动相为0.5 mL·L^(-1)磷酸溶液(A)-乙腈(B),梯度洗脱;流速:1.0mL·min^(-1);检测波长:203nm;柱温:30℃。结果人参皂苷Rg_1和人参皂苷Re及人参皂苷Rb_1在2.5~500μg·mL^(-1)、拟人参皂苷F11在5.0~1 000μg·mL^(-1)范围内线性关系良好(r≥0.999 4);平均加样回收率均大于95.0%。结论该方法简便、准确,重复性好,可作为西洋参含片的质量控制方法。Objective To establish an RP-HPLC method for simultaneous determination of four components in Panax quinquefolius L.Tablets,including gensenoside Rg1,gensenoside Re,gensenoside Rb1 and pseuoginsenoside F11.Methods The analysis was performed on an Agilent TC-C18column（250mm×4.6mm,5μm）with 0.5mL·L^-1 phosphoric acid-acetonitrile with step gradient elution.The flow rate was 1.0mL·min^-1 with detection wavelength of 203 nm.The column temperature was kept at 30℃.Results The ginsenoside Rg_1,ginsenoside Re,ginsenoside Rb1 and pseuoginsenoside F11 showed good linear relationship in the ranges of 2.5-500.0,2.5-500.0,2.5-500.0and 5.0-1 000.0μg·mL-1,respectively.All of the average recovery rates were more than 95.0%.Conclusion This method is simple,accurate,reliable and reproducible.It can be used for the quality control of Panax quinquefolius L.Tablets.

关 键 词：西洋参 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 拟人参皂F11 

分 类 号：R927.2[医药卫生—药学]