Occurrence and molecular characterization of Potato spindle tuber viroid(PSTVd) isolates from potato plants in North China  

作　　者：QIU Cai-ling ZHANG Zhi-xiang LI Shi-fang BAI Yan-ju LIU Shang-wu FAN Guo-quan GAO Yan-ling ZHANG Wei ZHANG Shu Lü Wen-he Lü Dian-qiu 

机构地区：[1]Northeast Agricultural University, Harbin 150030, P.R. China [2]Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P,R, China [3]State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China

出　　处：《Journal of Integrative Agriculture》2016年第2期349-363,共15页农业科学学报（英文版）

基　　金：supported by the China Agriculture Research System(CARS-10-P14);the National Science Foundation for Post-doctoral Scientists of China(20110491125);the Heilongjiang Funds for Distinguished Young Scientist,China (JC 201018);the Harbin Application Technology Research and Development Projects,China(2013AE6AW059);the Young Scientists Science Foundation of Heilongjiang Province,China(QC2015026)

摘　　要：China is the largest potato producing country worldwide,with this crop representing the fourth largest staple food crop in China.However,the steady presence of Potato spindle tuber viroid（PSTVd） over the past five decades has a significant economic impact on potato production.To determine why PSTVd control measures have been ineffective in China,more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality,Ministry of Agriculture,China.A high PSTVd infection rate（6.5%） was detected among these commercial seed potatoes.Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection,revealing a high rate of PSTVd contamination in these potato propagation materials.Furthermore,comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants,which represented 42 different sequence variants of PSTVd.Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China.Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia.It is worth noting that three intermediate strains and six mild strains were identified among these variants.These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.China is the largest potato producing country worldwide,with this crop representing the fourth largest staple food crop in China.However,the steady presence of Potato spindle tuber viroid（PSTVd） over the past five decades has a significant economic impact on potato production.To determine why PSTVd control measures have been ineffective in China,more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality,Ministry of Agriculture,China.A high PSTVd infection rate（6.5%） was detected among these commercial seed potatoes.Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection,revealing a high rate of PSTVd contamination in these potato propagation materials.Furthermore,comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants,which represented 42 different sequence variants of PSTVd.Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China.Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia.It is worth noting that three intermediate strains and six mild strains were identified among these variants.These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.

关 键 词：certification germplasm phylogenetic analysis quarantine seed potato viroid 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] S858.31[农业科学—植物保护]