鬼针草总黄酮对过敏性紫癜患儿血清IgA1诱导血管内皮细胞损伤的保护作用  

Protective Effect of Total Flavones of Bidens pllosa L.on IgA1 Induced Injury of HUVECs in Henoch-Schonlein Purpura Children Patients

作　　者：费文君[1] 袁丽萍[2] 鹿玲[2] 桂金贵[1] 

机构地区：[1]安徽中医药大学第一附属医院儿科,合肥230031 [2]安徽医科大学第一附属医院儿科,合肥230022

出　　处：《中国中西医结合杂志》2016年第2期183-187,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家青年自然科学基金资助项目(No.81001339)

摘　　要：目的探讨鬼针草总黄酮(total flavones of Bidens pi/osa L.,TFB)对过敏性紫癜(HenochSch(o|¨)nlein purpura,HSP)患儿血清IgA1诱导血管内皮细胞损伤的保护作用及机制。方法以人脐静脉内皮细胞(human umbilical venous endothelial cells,HUVECs)为研究对象,用正常IgA1和HSP患儿血清IgA1进行干预,同时加入不同浓度TFB,分为空白对照组、正常对照组、HSP IgA1组及TFB(1.0、0.5、0.25 mg/mL)组。ELISA法分别检测各组上清液IL-8和TNF-α水平,硝酸还原酶法检测各组上清液NO水平;荧光定量PCR和Western blot法检测HUVECs中NF-κB和ICAM-1 mRNA和蛋白表达。结果与正常对照组和空白对照组比较,HSP组IL-8、TNF-α及NO水平均明显升高,差异有统计学意义(P<0.05);与HSP组比较,TFB(1.0、0.5 mg/mL)干预后IL-8、TNF-α及NO水平均明显降低,差异有统计学意义(P<0.05,P<0.01)。荧光定量PCR和Western blot检测结果均显示:与正常对照组和空白对照组比较,HSP患儿血清IgA1诱导内皮细胞NF-κB与ICAM-1 mRNA和蛋白表达水平明显升高,差异有统计学意义(P<0.05,P<0.01);与HSP组比较,TFB 1.0,0.5、0.25 mg/mL干预下,NF-κB与ICAM-1mRNA和蛋白表达明显下调,差异有统计学意义(P<0.05,P<0.01)。结论 TFB可能通过抑制性HSP患儿体内NF-κB高表达、减少血管内皮细胞分泌IL-8、TNF-α、NO而缓解血管损伤发生。Objective To explore the protective effect and mechanism of total flavones of Bidens pilosa L.（TFB） on lgA1 induced injury of venous endothelial cells in Henoch-Sch（o｜¨）nlein purpura（HSP）children patients.Methods Human umbilical venous endothelial cells（HUVECs） were taken as subject.They were intervened by normal lgA1 and HSP children patients’ serum lgA1,and added with different concentrations TFB at the same time.Then they were divided into the blank control group,the normal control group,the HSP lgA1 group,and HSP lgA1 plus TFB（1.0,0.5,0.25 mg/mL） groups.Levels of TNF-α and IL-8 in supernate were detected by ELISA.The NO level was detected by nitrate reductase method.mRNA and protein expressions of NF-kB and ICAM-1 in HUVECs were detected by fluorescent quantitative PCR and Western blot respectively.Results Compared with the normal control group and the blank control group,levels of IL-8,TNF-α,and NO all significantly increased in the HSP group（P 〈0.05）.Compared with the HSP group,levels of IL-8,TNF-a,and NO significantly decreased after intervention of TFB（1.0 and 0.5 mg/mL;P〈0.05,P〈0.01）.Results of fluorescent quantitative PCR and Western blot showed,as compared with the blank control group and the normal control group,mRNA and protein expressions of NF-kB and ICAM-1 in HSP children patients’ serum lgA1 induced venous endothelial cells significantly increased with statistical difference（P〈0.05,P〈0.01）.Compared with the HSP group,mRNA and protein expressions of NF-κB and ICAM-1 were obviously down-regulated after intervention of TFB（1.0,0.5,0.25 mg/mL）,with statistical difference（P〈0.05,P〈0.01）.Conclusion TFB could protect vascular damage by inhibiting in vivo high expression of NF-κB,reducing the production of IL-8,TNF-α,and NO in vascular endothelial cells of HSP children patients.

关 键 词：过敏性紫癜 人脐静脉内皮细胞 细胞因子 鬼针草总黄酮 

分 类 号：R285.6[医药卫生—中药学]