五味子提取物对苯并[a]芘暴露致妊娠早期大鼠胚胎损伤的保护作用  

Protective Effect of Schisandra Extract on Embryotoxicity and Reproductive Toxicity in Early Pregnant Rats Exposed to Benzo[a]pyrene

作　　者：梁婧[1,2] 侯海燕[1,3] 孙旸[1] 陈亚琼[1,3] 

机构地区：[1]武警后勤学院附属医院妇产科,天津300162 [2]天津中医药大学研究生院,天津300193 [3]天津市职业与环境危害防制重点实验室,天津300162

出　　处：《中国中西医结合杂志》2016年第2期234-238,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.81273977);天津市自然科学基金一般项目(No.12JCYBJC16200)

摘　　要：目的观察五味子提取物对妊娠期苯并[a]芘(Benzo[a]pyrene,Bap)暴露致早孕期大鼠胚胎损伤的防治作用。方法采用性周期筛选合笼法制备孕鼠模型。将50只SD雌性孕鼠按体重进行随机区组设计分为BaP模型组、五味子低、中、高剂量组和正常对照组,每组10只。BaP模型组予BaP 2 mg/(kg·d)灌胃,五味子低、中、高剂量组分别予五味子提取物40、200、1 000 mg/(kg·d)加BaP 2 mg/(kg·d)灌胃,正常对照组予相同体积的橄榄油,持续灌胃8天。观察大鼠各期体重变化,子宫连胚总质量、卵巢质量并计算脏器指数;统计黄体数、胚胎着床数、吸收胎数并计算着床率和吸收胎率;E LISA法检测大鼠血清绒毛膜促性腺激素(human chorionic gonadotrophinβ,β-HCG)及孕酮(progesterone,PROG)水平。结果与正常对照组比较,Bap模型组大鼠孕9天体重、胚胎着床数、子宫连胚总指数、卵巢指数、大鼠血清β-CG及PROG水平均下降,差异有统计学意义(P<0.05,P<0.01);与Bap模型组比较,五味子低、中及高剂量组大鼠体重、子宫连胚总指数及PROG水平增加(P<0.05,P<0.01),五味子中、高剂量组大鼠卵巢指数及血清β-HCG水平升高(P<0.05,P<0.01),五味子高剂量组大鼠胚胎着床数明显增高(P<0.01)。结论五味子提取物可以降低妊娠期苯并[a]芘暴露对大鼠胚胎及生殖造成的毒性作用。Objective To observe protective effects of Schisandra extract（SE） on embryotoxicity and reproductive toxicity of early pregnant rats exposed to Benzo[a]pyrene（Bap）.Methods Pregnant rat model was prepared using periodic screening cage method.Totally 50 female pregnant SD rats were divided into five groups by randomized block design according to the weight,i.e.,the BaP model group,the low dose SE group,the middle dose SE group,the high dose SE group,the normal control group,10 rats in each group.Rats in the BaP model group were administered with BaP at a daily dose of2 mg/kg by gastrogavage.Rats in low,middle,and high dose SE groups were administered by gastrogavage with BaP（at a daily dose of 2 mg/kg） plus SE at a daily dose of 40,200,and 1 000 mg/kg,respectively.Equal volume of olive oil was administered to rats in the normal control group by gastrogavage.All medication was performed for 8 successive days.Changes of rat body weight in each period were observed.The uterus embryonic total quality and ovary quality were measured,and organ index calculated.The number of corpus luteum,the number of embryo implantation,and the number of absorbed embryo were statistically calculated respectively.The implantation rate and the absorbed embryos rate were calculated.Serum levels of human chorionic gonadotrophin β（β-HCG） and progesterone（PROG） were detected by ELISA.Results Compared with the normal control group,the weight of 9-day pregnant rats,the number of embryo implantation,the uterus embryonic total index,ovary index,serum levels of β-HCG and PROG all decreased in the Bap model group with significant difference（P 〈0.05,P 〈0.01）.Compared with the Bap model group,body weight,the uterus embryonic total index,and the PROG level increased in 3 dose SE groups（P 〈0.05,P 〈0.01）.Ovary index and serum β-HCG increased in middle and high dose SE groups（P 〈0.05,P 〈0.01）.The number of implantation obviously increased in the high dose SE groups（P 〈0.01）.Conclusion SE could reduce

关 键 词：五味子提取物 苯并[A]芘 早孕期 胚胎毒性 保护作用 

分 类 号：R285.5[医药卫生—中药学]