1800MHz电磁辐射对小鼠皮肤细胞的氧化损伤作用研究  

Oxidative damage of 1800 MHz electromagnetic radiation on mouse epidermis cells

作　　者：刘欢[1] 王明连[1] 马雪梅[1] 林太凤[1] 刘巧丽[1] 李艳[1] 

机构地区：[1]北京工业大学,北京100124

出　　处：《中国预防医学杂志》2016年第1期19-22,共4页Chinese Preventive Medicine

基　　金：北京市教育委员会科技计划面上项目(2011CB503705)

摘　　要：目的研究1 800 MHz电磁辐射对细胞的氧化损伤作用。方法使用1 800 MHz,比吸收率为2、3、4 W/kg电磁辐射辐照小鼠皮肤细胞(JB6),细胞分为辐照组与对照组。辐照结束后,采用流式细胞术,DCFH-DA荧光探针检测细胞内活性氧(ROS)水平,再加入ROS中和剂富氢水,以观察其对ROS的中和作用。通过硫代巴比妥酸比色法检测丙二醛含量,来评价电磁辐射后细胞氧化损伤程度。结果比吸收率为2、3 W/kg时,暴露2h内细胞内ROS水平变化差异无统计学意义;4 W/kg暴露20 min、80 min、120min后,暴露组细胞与假暴露组比较ROS水平均显著升高(P<0.05);加入富氢水后,暴露组与普通培养基暴露组比较,降低了50.79%;4 W/kg暴露120min后丙二醛含量显著升高(P<0.05)。结论4 W/kg 1 800 MHz电磁辐射会引发细胞内ROS升高并造成细胞氧化损伤。Objective To observe the potential oxidative damage of 1 800 MHz intermittent electromagnetic fields（EMF）on mouse epidermis cells. Methods JB6 mouse epidermis cells were exposed to 1 800 MHz EMF at SAR of 2,3,4W/kg for 2h.Flow cytometry and DCFH-DA fluorescence probe were used to determine the ROS level.The hydrogen-rich saline was then added into the cell culture medium in order to observe the hydrogen protection for cells from ROS.Thiobarbituric acid（TBA）was used to determine the level of malondialdehyde,which reflected the level of oxidative damage of cells. Results The changes of ROS of cells were not observed when exposed to SAR of 2 W/kg and 3 W/kg.While,the ROS levels of cells increased significantly when exposed to SAR of 4 W/kg for 20 min,80min,120 min compared to those in sham group（P〈0.05）.After addition of hydrogen-rich medium,the level of ROS was similar to that of sham group and decreased by 50.79%.Meanwhile,MDA level increased only when cells were exposed to SAR of 4 W/kg for 120 min. Conclusions1 800 MHz EMF cell exposure at 4W/kg can increase the level of ROS and induce ROS-mediated oxidative stress injury.

关 键 词：电磁辐射 氧化损伤 活性氧(ROS) 丙二醛(MDA) 富氢水 

分 类 号：R144[医药卫生—公共卫生与预防医学]