吡咯喹啉醌对高脂血症大鼠糖脂代谢肝功能及脂质过氧化影响的实验研究  

Effects of PQQ on serum lipid and glucose levels,liver function and lipid peroxidation in hyperlipidemia rats

作　　者：胡文敏[1] 李林子[1] 张岭[1] 张丽婧[1] 陈建国[1] 刘冬英[1] 刘臻[1] 胡志航[1] 王茵[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《中国预防医学杂志》2016年第1期23-27,共5页Chinese Preventive Medicine

基　　金：浙江省医药卫生科技计划项目(2011ZDA001;2014ZDA004);浙江省营养学医学支撑学科建设项目(11-zc03);浙江省科技计划项目(2011F20038;2013F10007;2007C12019);浙江省151人才工程项目

摘　　要：目的探讨吡咯喹啉醌(PQQ)对实验性高脂血症大鼠血脂、血糖、肝功能及脂质过氧化的影响。方法将SD大鼠随机分为正常组和高脂造模组,正常组饲予基础饲料,高脂造模组饲予高糖高脂饲料5个月建立高脂血症模型,从中挑选出40只高脂血症大鼠,按血脂水平随机分为模型对照组、PQQ低剂量组、PQQ中剂量组和PQQ高剂量组,每组10只,分别灌胃给予15、30、60mg/kg PQQ,连续6周,检测血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、血糖(GLU)、胰岛素(INS)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px),并对肝脏做病理组织学检查。结果 PQQ可降低高脂血症大鼠血清中TC、TG、LDL-C、ALT、AST水平,升高HDL-C/TC比值;同时能提高血清中SOD、GSH-Px的活力,降低MDA水平,并且能降低血清中血糖和胰岛素抵抗水平。结论 PQQ可以调节高脂血症模型大鼠血脂、血糖水平和因高血脂导致的肝损伤,同时具有一定的抵抗脂质过氧化作用,提示其对预防高脂血症具有积极作用。Objective To explore the effects of pyrroloquinoline quinine（PQQ）on serum lipid and glucose levels,liver function and lipid peroxidation in hyperlipidemia rats. Methods Spraggs Dawley rat models with hyperlipidemia were induced by feeding them with high-sugar and high-fat diet for 5months.Forty hyperlipidemia rats were selected and evenly divided into 4groups and were given intragastrically PQQ of 0,15,30or60mg/kg for 6weeks.Serum levels of total cholesterol（TC）,triglycerides（TG）,high-density lipoprotein cholesterol（HDL-C）,glucose（GLU）,insulin（INS）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,oxide diastase（SOD）,paraformaldehyde（MDA）and glutathione peroxidase（GSH-Px）were measured and pathological and histopathological changes of livers were evaluated. Results Serum levels of TC,TG,LDL-C,ALT,AST and MDA decreased,while HDL-C/TC ratio,SOD and GSH-Px activity increased in hyperlipidemia rats who received PQQ.Meanwhile,glucose and insulin resistance also decreased in those rats. Conclusions PQQ may regulate serum levels of lipid and glucose,improve hepatic damage and slow down lipid peroxidation in hyperlipidemia rats.Therefore,it may be potentially used in the prevention and treatment of hyperlipidemia.

关 键 词：吡咯喹啉醌 高脂血症 血脂 血糖 脂质过氧化 

分 类 号：R589.2[医药卫生—内分泌]