白花丹醌对人肝星状细胞的细胞毒性及其机制的研究  

The Research on toxicity and its mechanism of human hepatic stellate cells by Plumbagin

作　　者：彭岳[1,2] 苗维纳[1] 赵铁建[2] 黎桂玉 

机构地区：[1]成都中医药大学基础医学院,四川成都610075 [2]广西中医药大学基础医学院,广西南宁530200

出　　处：《时珍国医国药》2016年第1期5-7,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金地区项目(No.81160553);国家自然科学基金青年项目(No.81403189);国家自然科学基金地区项目(No.81460628);广西教育厅研究生教育创新计划基金项目(No.JGY2014082)

摘　　要：目的研究白花丹醌对人肝星状细胞株(HSC-LX2)的细胞毒性作用,并通过细胞周期及凋亡率的变化探讨该药抑制细胞增殖的机制。方法 HSC-LX2与药物孵育48h,以MTT法检测细胞毒性;以流式细胞术PI染色法检测各周期细胞DNA的含量及细胞晚期凋亡率。结果白花丹醌低、中、高浓度对HSC-LX2细胞均具有明显的细胞毒性并抑制其细胞增殖;G_0/G_1期细胞的比例发生升高而S期+G_2/M期细胞总数降低;且细胞的晚期凋亡率明显增高。以上作用具有剂量依赖性,有统计学差异(P<0.05)。结论白花丹醌对HSC-LX2具有明显的细胞毒性而对肝纤维化进程有干预作用,原因为将细胞周期阻滞在G_0/G_1期而阻止细胞的分裂增殖,并能诱导细胞发生凋亡。上述能力均有剂量依赖性;且中、高剂量白花丹醌的干预能力强于秋水仙碱和复方鳖甲软肝片。Objective To study Plumbagin on human hepatic stellate cells（ HSC- LX2） cytotoxicity. By observing the changes of cell cycle and apoptosis rates,investigate the mechanism of drug inhibition of cell proliferation. Methods HSC- LX2 drug incubation 48 h,cytotoxicity by MTT assay; PI flow cytometry staining content and late apoptosis rate of each cell cycle DNA. Results Low,medium and high concentrations of Plumbagin of HSC- LX2 cells have significant cytotoxic effect and can inhibit the proliferation of HSC- LX2 cells; and so the proportion of G_0/ G_1 phase of the cell increases occurred at the same time S phase ＋G_2/ M phase of the total number of cells reduced; the drug can make late apoptotic rate HSC- LX2 cells significantly increased.These effects are dose dependent,statistically significant（ P〈0. 05）. Conclusion Plumbagin on HSC- LX2 has significant cytotoxicity of liver fibrosis have rats. The reason is that the drug will HSC- LX2 cell cycle arrest in G_0/ G_1 phase and prevent cell proliferation and induce apoptosis. These capabilities are dose- dependent manner; and the ability to intervene in high doses Plumbagin stronger than colchicine and Fufangbiejiarangan film.

关 键 词：白花丹醌 人肝星状细胞 细胞毒性 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]