蒙药塔日奴中岩大戟内酯B和狼毒乙素的RP-HPLC法分析  

Simultaneous determination of jolkinolide B and 2,4-dihydroxy-6-methoxy-3-methy-1-acetophenone in Mongolian medicine Tarinu by RP-HPLC

作　　者：许良[1] 包勿兰 李桂花[1] 王曦烨[1] 刘景林[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028000

出　　处：《时珍国医国药》2016年第1期19-21,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260682);精细化工国家重点实验室开放课题(No.KF1308)

摘　　要：目的建立反相高效液相法同时测定蒙药塔日奴中岩大戟内酯B和狼毒乙素含量。方法采用90%的乙醇溶液回流提取制备供试品。分析色谱柱为Century SIL C_(18)BDS(250 mm×4.6 mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱(0~60 min;98%~0%B;2%~100%A),流速0.8 ml·min^(-1),检测波长227 nm。结果蒙药塔日奴中岩大戟内酯B及狼毒乙素标准曲线的回归方程依次为Y=458411X+1704565(r=0.9999),Y=127938X-168409(r=0.9998),在1.871~9.355μg,0.1856~0.928μg范围内线性关系良好,平均回收率分别为99.5%和95.5%,RSD分别为3.2%和1.0%。结论所建立的HPLC含量测定法快捷、准确、重现性好,可用于蒙药塔日奴中岩大戟内酯B和狼毒乙素的含量测定。Objective To develop a RP- HPLC method for the simultaneous determination of jolkinolide B and 2,4- dihydroxy- 6- methoxy- 3- methy- 1- acetophenone in Mongolian herbal medicine Tarinu. Methods The 90 % ethanol reflux extraction method was used to extract two components. The separation was performed on A Century SIL C_（18） BDS（ 250 mm × 4. 6 mm,μm） column. The mobile phase consisted of acetonitrile and water with gradient elution（ 0 ~ 60 min; 98 % ~ 0 % B; 2 % ~ 100% A）,at the flow rate of 0. 8 ml·min^（-1）and the detection wavelength was 227 nm. Results The regression equations of the two components in Mongolian medicine Tarinu were Y = 458411 X ＋ 1704565（ r = 0. 9999） for jolkinolide B,Y = 127938X- 168409（ r = 0. 9998）,for 2,4- dihydroxy- 6- methoxy- 3- methy- 1- acetophenone,respectively. The calibration curves of two components,jolkinolide B and 2,4- dihydroxy- 6- methoxy- 3- methy- 1- acetophenone were linear in the ranges of 1. 871~ 9. 355 μg and 0. 1856 ~ 0. 928 μg,the average recoveries were 99. 5 % with RSD of 3. 2 % for jolkinolide B and 95. 5 %with RSD of 1. 0 % for 2,4- dihydroxy- 6- methoxy- 3- methy- 1- acetophenone. Conclusion The determination method of two components in Mongolian medicine Tarinu was fast,accuracy,good reproducibility and could be used for the quality control of Mongolian herbal medicine Tarinu。

关 键 词：岩大戟内酯B 狼毒乙素 反相高效液相色谱法 蒙药塔日奴 

分 类 号：R291.2[医药卫生—民族医学]