天然沉香中白木香酸含量的高效液相色谱法测定  

Determination of Agaropiric Acid in Natural Agarwood by HPLC

作　　者：钟兆健[1] 樊云飞[1] 雷智东 潘清灵[3] 周欣[1] 刘岱琳[2] 章卫民[3] 高晓霞[1] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]武警后勤学院,天津300309 [3]广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《时珍国医国药》2016年第1期21-24,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81102418);广东省基础与应用基础研究专项资金(省自然科学基金)(No.2014A030313583);广东省科技计划项目(No.2012A030100014);广东省质量技术监督局标准制修订计划项目(No.粤质监标函[2012]676号);广东省科学院分析测试基金项目(No.Sf212906)

摘　　要：目的建立天然沉香中白木香酸含量的分析方法,研究白木香酸与浸出物含量(%)之间的相关性。方法按《中国药典》(2010年版)一部进行醇溶性浸出物的含量测定和理化鉴别。高效液相色谱法采用CNW Athena C_(18)-WP柱(250mm×4.6mm,5μm),以乙腈-0.1%(体积分数)磷酸水溶液(40∶60)为流动相,流速1.0ml·min^(-1),检测波长217nm,柱温35℃。结果白木香酸在2.000~80.00μg·ml^(-1)(r=0.9999)范围内线性关系良好,平均回收率为96.1%,RSD为3.0%(n=6)。在理化鉴别和浸出物含量合格的情况下,天然沉香中白木香酸与浸出物含量之间呈正比关系。结论高效液相色谱法可准确测定天然沉香中白木香酸的含量,结合白木香酸与浸出物含量之间的相关性,为天然沉香的质量评价提供依据。Objective To establish a HPLC method for determination of agaropiric acid in natural agarwood and research the relativity of contents between the ethanol- soluble extraction and agaropiric acid. Methods According to those correlative standards of Aquilariae Lignum Resinatum in Ch. P.（ 2010 Edith）,the content of ethanol- soluble extraction were determined and the physicochemical properties of samples were identified. The determination was performed on a CNW Athena C_（18）- WP column（ 250 mm × 4. 6 mm,5μm） and the mobile phase consisted of acetonitrile- 0. 1% phosphoric acid（ 40： 60）. The flow rate was1. 0 ml·min^（-1）. The detection wave length was 217 nm and the column temperature was 35℃. Results The good linear range of agaropiric acid was 2. 000 ~ 80. 00 g·ml^（-1）（ r = 0. 9999）. The average recovery was 96. 1% with RSD = 3. 0%（ n = 6）. Under the circumstance that the content of ethanol- soluble extraction and the physicochemical properties of samples were qualified,the relation of contents between the ethanol- soluble extraction and agaropiric acid of natural agarwoods was directly proportional.Conclusion This method is accurate based on HPLC determination of agaropiric acid. The relativity of contents between the ethanol- soluble extraction and agaropiric acid provided a reference to quality evaluation of natural agarwood.

关 键 词：天然沉香 白木香酸 浸出物 

分 类 号：R284.2[医药卫生—中药学]