针刺对慢性情绪应激焦虑大鼠肾上腺ANP、CNP及血浆CORT水平的影响  

Effects of acupuncture on ANP and CNP in adrenal gland and CORT in plasma in rats with chronic emotional stress anxiety

作　　者：姚弘[1] 魏大能[1] 蔡定均[1] 余曙光[1] 张承舜[1] 魏京金 肖夏[1] 周奇志[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《中国针灸》2016年第2期169-174,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81173322;教育部博士点基金博导类课题项目:20135132110009

摘　　要：目的:分析针刺对慢性情绪应激焦虑大鼠肾上腺心房利钠肽(atrial natriuretic peptide,ANP)、C-型利钠肽(C-type natriuretic peptide,CNP)水平及血浆皮质酮(corticosterone,CORT)含量的影响,探讨针刺治疗焦虑障碍的部分作用机制。方法:选用健康Sprague-Dawley(SD)大鼠32只,经7d饲养驯化后,随机分为3组,即空白组(10只)、模型组(11只)、针刺组(11只)。模型组、针刺组运用不可预知的慢性情绪应激刺激(chronic emotion stress,CES)方法,建立CES焦虑模型,针刺组同时针刺"内关""神门",隔日1次,每次30min,造模和治疗共计15d。空白组相同方法固定,不予处理。运用高架十字迷宫测试焦虑大鼠的行为学变化,酶联免疫法检测血浆CORT含量,免疫组化法检测肾上腺皮质和髓质ANP、CNP的表达水平。结果:(1)行为学方面,模型组在高架十字迷宫开臂滞留的时间(open-arms time,OT)占开臂和闭臂滞留总时间的百分比(OT%值)较空白组明显下降(P<0.05),针刺组OT%值较模型组有明显升高(P<0.05);(2)血浆CORT含量,模型组较空白组明显升高(P<0.05),而针刺组与模型组相比明显降低(P<0.05);(3)与空白组相比,模型组大鼠肾上腺皮质、髓质ANP表达水平降低(P<0.01)、CNP水平升高(P<0.01),针刺组较模型组肾上腺髓质ANP水平升高(P<0.05)、CNP水平降低(P<0.01)。结论:针刺抗焦虑作用的发挥可能与其调节外周肾上腺髓质ANP和CNP的表达水平,进而影响CORT的释放、抑制下丘脑-垂体-肾上腺轴(HPA)轴的活性有关。Objective To analyze the effects of acupuncture on the level of atrial natriuretic peptide（ANP）,Ctype natriuretic peptide（CNP）in adrenal gland and the content of corticosterone（CORT）in plasma in rats with chronic emotional stress anxiety,and to explore the partial action mechanism of acupuncture on anxiety disorder.Methods Thirty-two healthy Sprague-Dawley（SD）rats,after 7 days of feeding and domestication,were randomly divided into a blank group（10 rats）,a model group（11 rats）and an acupuncture group（11 rats）.The rats in the model group and acupuncture group were treated with unpredictable chronic emotional stress（CES）method to establish the model of anxiety.Rats in the acupuncture group were treated with acupuncture at＂Neiguan＂（PC 6）and ＂Shenmen＂（HT 7）,once every other day,30 minutes each time.The model establishment and treatment lasted 15 days.Rats in the blank group were treated with identical immobilization but no treatment was given.The elevated plus maze was used to test the behavioral changes of rats with anxiety;the level of CORT in plasma was detected by ELISA,and the expression level of CNP and ANP in adrenal cortex and medulla was detected by immunohistochemical method.Results（1）The percentage of open-arms time in total time（OT%）in elevated plus maze in the model group was significantly lower than that in the blank group（P〈0.05）;the OT%in the acupuncture group was significantly higher than that in the model group（P〈0.05）.（2）The content of CORT in plasma in the model group was higher than that in the blank group（P〈0.05）,while that in the acupuncture group was significantly lower than that in the model group（P〈0.05）.（3）The expression of ANP in adrenal medulla and cortex in the model group was lower than that in the blank group（P〈0.01）,while the expression of CNP in adrenal medulla and cortex in the model group was higher than that in the blank group（P〈0.01）.The expression of ANP in adrenal medulla in the

关 键 词：焦虑 针刺 肾上腺 心房利钠肽(ANP) C-型利钠肽(CNP) 

分 类 号：R246.6[医药卫生—针灸推拿学]