抗生素保存褐马鸡粪便分泌型免疫球蛋白A的时效性研究  

Effects of Antibiotics on the Storage of Fecal Secretory Immunoglobulin A of Brown-eared Pheasant

作　　者：周冉[1] 齐磊[1] 王文霞[1] 胡德夫[1] 隋金玲[1] 

机构地区：[1]北京林业大学自然保护区学院,北京100083

出　　处：《四川动物》2016年第1期58-61,共4页Sichuan Journal of Zoology

基　　金：全国第二次陆生野生动物资源调查--北京市常规调查项目(YBZ2015-003)

摘　　要：粪便免疫球蛋白是衡量野生动物健康状况及生存状态的有效手段之一,其中粪便的保存问题是关键。为探索抗生素对粪便分泌型免疫球蛋白A(SIg A)的保存时效性,采用酶联免疫吸附法对4种常用抗生素(利福平、四环素、卡那霉素和庆大霉素)的3种不同浓度(1μg·m L^(-1)、25μg·m L^(-1)、50μg·m L^(-1))保存的褐马鸡Crossoptilon mantchuricum粪便SIg A的含量进行了测定。结果表明,25μg·m L^(-1)的利福平保存的褐马鸡粪便SIg A效果最佳,其次是50μg·m L^(-1)的四环素和50μg·m L^(-1)的卡那霉素,庆大霉素保存效果最差。Evaluation of fecal immunoglobulin level is an effective means of monitoring health and survival situation in wildlife,and this method requires the storage of feces in an appropriate way. In the present study,four antibiotics including rifampicin,tetracycline,kanamycin and gentamicin were used to investigate the efficiency for preservation of fecal secretory immunoglobulin A（ SIg A） of brown-eared pheasant（ Crossoptilon mantchuricum） at three different concentrations（ 1 μg·m L^（-1）,25 μg·m L^（-1）,50 μg·m L^（-1））. The content of SIg A was measured by enzyme linked immunosorbent assay. The results showed that rifampicin at concentration of 25 μg · m L^（-1）was optimal for SIg A preservation,followed by50 μg·m L^（-1）tetracycline and 50 μg·m L^（-1）kanamycin,whereas the storage effect of gentamicin was the worst.

关 键 词：褐马鸡 抗生素 粪便分泌型免疫球蛋白A 保存时效 

分 类 号：S865[农业科学—野生动物驯养]