长足大竹象雄性附腺提取物抑菌作用研究  

Study on Antibacterial Activity of Male Accessory-gland Extracts from Cyrtotrachelus buqueti

作　　者：梁梓[1] 杜超豪[1] 杨瑶君[1] 农向[1] 廖鸿[1] 颜珊[1] 

机构地区：[1]乐山师范学院生命科学学院,四川乐山614004

出　　处：《四川动物》2016年第1期66-69,共4页Sichuan Journal of Zoology

基　　金：四川省教育厅项目(13ZB0112)

摘　　要：以3种细菌(大肠杆菌Escherichia coli、金黄色葡萄球菌Bacillus subtilis、枯草芽孢杆菌Staphyloccocus aureus)为供试菌,测定了长足大竹象Cyrtotrachelus buqueti雄性附腺提取物的抑菌活性。结果表明:长足大竹象雄性附腺提取物对革兰氏阳性菌如枯草芽孢杆菌、金黄色葡萄球菌均有一定的抑菌活性。不同浓度的粗提物对枯草芽孢杆菌及金黄色葡萄球菌抑菌影响差异均具有高度统计学意义(P<0.01),抑菌活性随着粗提物浓度的增加而增强。粗提物对金黄色葡萄球菌和枯草芽孢杆菌的最小抑菌浓度分别为0.2 mg·m L^(-1)、0.4 mg·m L^(-1)。不同处理温度对长足大竹象雄性附腺粗提物的抑菌作用有一定影响。The antibacterial activities of male accessory-gland extracts from Cyrtotrachelus buqueti were determined using three species of bacteria（ Escherichia coli,Bacillus subtilis,and Staphyloccocus aureus）. The results showed that male accessory-gland extracts from C. buqueti had a certain antibacterial activity on the Gram-positive bacteria such as B. subtilis and S. aureus,and the antibacterial activities were significantly differed among different concentrations of male accessorygland extracts. The antibacterial activity would increase with increasing concentrations of the extracts. The minimum inhibitory concentration of male accessory-gland extracts against B. subtilis and S. aureus were 0. 2 mg · m L^（- 1）and0. 4 mg·m L^（- 1）,respectively. Temperature also had a certain influence on the antibacterial effects of male accessory-gland extracts from C. buqueti.

关 键 词：长足大竹象 雄性附腺 抑菌作用 

分 类 号：Q966[生物学—昆虫学]