上海市2007-2013年HIV-1分子流行病学演变趋势  

Molecular Epidemiological Characteristics of Human Immunodeficiency Virus Type 1 in Shanghai,2007-2013

作　　者：吴健[1] 王绪琴[2] 周艳秋[3] 郁晓磊 盖晶[2] 袁家麟[1] 郑敏[4] 陶静[5] 薛以乐[2] 康来仪[2] 

机构地区：[1]上海市黄浦区疾病预防控制中心,上海200023 [2]上海市疾病预防控制中心,上海200336 [3]上海市静安区疾病预防控制中心,上海200041 [4]上海市长宁区疾病预防控制中心,上海200051 [5]上海市虹口区疾病预防控制中心,上海200082

出　　处：《中国艾滋病性病》2016年第1期5-9,共5页Chinese Journal of Aids & STD

基　　金：国家"十二五"科技重大专项(2012ZX10001-002-002-012)~~

摘　　要：目的了解上海市2007-2013年艾滋病病毒(HIV)-1亚型流行趋势。方法选取2007-2013年确认的、首次随访的HIV-l感染者1296例。取其血浆标本,应用反转录-聚合酶链反应(RT-PCR)扩增,DNA测序,系统进化树分析,并结合流行病学资料进行分析。结果获得1085例pol区基因片段,其中亚型分布显示,CRF01_AE所占比例为59.1%(641例),CRF07_BC为23.1%(251例),B亚型为9.3%(101例),CRF08_BC为4.2%(46例),C亚型为1.1%(12例),CRF55_01B为0.9%(10例),CRF59_01B为0.8%(9例),CRF02_AG为0.6%(6例),CRF58_01B为0.3%(3例),未定型CRF_01B为0.3%(3例),CRF67_01B为0.2%(2例)和G亚型为0.1%(1例)。HIV-1主要流行毒株,CRF01_AE的比例从2007年64.3%下降到2013年的55.2%,CRF07_BC从2007年的8.6%上升到2013年的30.2%。CRF01_AE、CRF07_BC和B亚型均以性传播途径为主,其中同性性传播分别为61.3%(393/641)、49.8%(125/251)、51.5%(52/101),异性性传播分别为36.3%(233/641)、35.5%(89/251)、44.6%(45/101);CRF08_BC中异性性传播占60.9%(28/46);C亚型、CRF55_01B、CRF59_01B和CRF58_01B均以性传播为主;CRF02_AG以异性性传播为主,未定型CRF01_B、CRF67_01B和G亚型均以同性性传播为主。结论上海市2007-2013年HIV-1亚型变化明显,目前以CRF01_AE和CRF07_BC为主。继续加强对HIV-1基因亚型监测具有重要意义。Objective To better understand the human immunodeficiency virus-1（HIV-1）phylogenetical tendency from 2007 to 2013in Shanghai.Methods A total of 1296 people with HIV-1-infection living in Shanghai,were randomly sampled and their plasma was collected.The samples were amplified by nest RT-PCR,DNA was sequenced and phylogenetically analyzed.All results were analyzed in combination with the data of epidemiological survey.Results With fragments of 1085pol-gene gathered,phylogenetical tendency showed that CRF01_AE was predominant by 59.1%（641）,and followed by CRF07_BC of 23.1%（251）,subtype B of 9.3%（101）,CRF08_BC of4.2%（46/1985）and subtype C of 1.1%（12）,CRF55_01of 0.9%（10）,CRF59_01Bof 0.8%（9）,CRF02_AG of 0.6%（6）,CRF58_01Bof 0.3%（3）,no subtypeCRF_01Bof 0.3%（3）,CRF67_01Bof 0.2%（2）,and subtype G of0.1%（1）respectively.CRF01_AE was decreased from 64.3% in 2007 to 55.2%in 2013,while CRF07_BC increased from 8.5%in 2007 to 30.2%in2013.CRF01_AE,CRF07_B and subtype B were spread by sexual transmission,with homosexuals by61.3%（393/641）,49.8%（125/251）,51.5%（52/101）respectively,and heterosexuals by 36.3%（233/641）,35.5%（89/251）,44.6%（45/101）;while CRF08_BC was mainly spread by heterosexual transmission,by 60.9%（28/46）,Subtype C,CRF55_01B,CRF59_01Band CRF58_01Bwere all spread by sexual transmission.CRF02_AGwas spread by heterosexual transmission.Not stereotyped CRF01_B,CRF67_01Band subtype G were all spread by homosexual transmission.Conclusion HIV-1phylogenetical change is apparent in Shanghai,and currently the priority subtypes are CRF01_AE and CRF07_BC.Continuing surveillance on HIV-1subtype gene-types is of great importance.

关 键 词：艾滋病病毒-1型 分子流行病学 演变趋势 

分 类 号：R373.9[医药卫生—病原生物学]