药品中诺氟沙星的量子点荧光猝灭测定法  

Determination of norfloxacin in drug by fluorescence quenching of CdTe quantum dots

作　　者：张媛[1] 谢海婷 高强[1] 李满秀[1] 

机构地区：[1]忻州师范学院化学系,忻州034000

出　　处：《中国抗生素杂志》2016年第2期125-127,共3页Chinese Journal of Antibiotics

基　　金：国家级大学生创新训练项目(No.20141012499);山西省大学生创新创业训练项目(No.2014382);忻州师范学院化学化工创新实践基地基金(2014)

摘　　要：目的建立药品中诺氟沙星的量子点荧光猝灭测定法。方法在诺氟沙星溶液中加入适量CdTe量子点溶液和N-羟基丁二酰亚胺（NHS）溶液，用pH=7．5硼砂缓冲溶液定容。混合均匀后在室温下反应30min，测定其荧光强度。结果诺氟沙星在1．91～35．09μg／mL范围与体系的F0／F呈良好的线性关系，相关系数r=0．9961，检出限为1．70gg／mL，平均回收率为93．52％-98．75％，RSD为1．1％-4．8％。结论方法具有快速简便，灵敏度和精密度较高的优点，适用于药品中诺氟沙星的检测。Objective To develop a novel method for norfloxacin determined based on the fluorescence quenching of CdTe QDs . Methods In the borax buffer medium of pH7.5 and in the presence of NHS, run for 30min in room temperature, norfloxacin quenching fluorescence of CdTe QDs were determined. Results the linear relationship between relative fluorescence intensity Fo/Fand concentration of norfloxacin is kept in the range of 1.91-35.09μg/mL. The correlation coefficient （r=0.9961）, the detection limit is 1.70μg/mL, the rates of recovery were 93.52%-98.75%. RSD were 1.1%-4.8%. Conclusion This method is sensitive, accurate and applicable to the determination of norfloxacin in drug samples with satisfactory results.

关 键 词：CDTE量子点 诺氟沙星 NHS 荧光猝灭 

分 类 号：R978.1[医药卫生—药品]