大鼠海马认知障碍对血糖血脂的影响及其胃肠激素水平的变化  

To investigate how the cognitive impairment in hippocampus of rats effects the glucose and lipid metabolism and the changes in gastrointestinal hormone levels

作　　者：叶琳[1] 朱琳[1] 杜森[1] 靳文雨 邵晓云[1] 王俊锋[1] 马军[1] 周思[1] 夏春波[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中风与神经疾病杂志》2016年第1期46-48,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.81260137)

摘　　要：目的探索大鼠海马认知障碍对血糖血脂的影响及其胃肠激素水平的变化,为神经内分泌学研究提供实验依据。方法将Aβ1-42注入大鼠海马制备大鼠认知障碍模型,检测大鼠血糖血脂水平,测定大鼠胃残留率及肠推进比,酶联免疫法检测大鼠血清及胃组织中胃动素(MTL)、胃泌素(GAS)、生长抑素(SS)的含量。结果(1)实验组大鼠血清中空腹血糖(FPG)、甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL)均明显高于假手术组和对照组(P<0.05)。(2)实验组大鼠胃残留率明显高于假手术组和对照组(P<0.05),实验组肠推进比明显低于假手术组和对照组(P<0.05)。(3)实验组大鼠血清中MTL、GAS、SS的含量及胃窦组织中SS的含量均明显高于假手术组和对照组(P<0.05),胃窦组织中MTL、GAS的含量较假手术组及对照组降低(P<0.05)。结论大鼠海马认知障碍可能会引起大鼠血糖血脂水平升高,其机制可能与胃肠激素的变化进而导致胃肠运动障碍有关。Objective To investigate the effects of cognitive imepairment in hippocampus of rats on glucose and lipid metabolism and the changes in gastrointestinal hormone levels,a series of experiments were established to provide experimental evidence for the study of the neuroendocrinology. Methods The Aβ1-42 was injected into the hippocampus of rats.The changes of the glucose and lipid levels were detected. Levels of gastrointestinal hormon in serum and gastric tissue were detected by ELISA. Results 1In the experimental group,FPG 、TG 、TC 、LDL were significantly higher than the sham group and the control group（ P 0. 05） 2Comparing with the sham group and the control group,gastric residual rate was significantly increased（ P 0. 05）,while alvine pushing rate was obviously decreased（ P 0. 05）. 3MTL、GAS、SS in serum and SS in gastric tissue were significantly higher in experimental group than other two groups（ P 0. 05）,while MTL and GAS in gastric tissue were significantly lower（ P 0. 05）. Conclusion Hippocampal cognitive impairment can cause the elevating of blood lipid levels,which may be related to the changes in gastrointestinal hormone levels and gastrointestinal motility disorders.

关 键 词：海马 糖脂代谢 胃肠激素 胃肠运动 

分 类 号：R338.26[医药卫生—人体生理学]