栀子药材及其炮制品的指纹图谱对比  

Comparation of HPLC Fingerprints between Fructus Gardeniae and Its Processed Products

作　　者：鲍家科 刘玲[2] 

机构地区：[1]贵州省药品审评认证中心,贵州贵阳550004 [2]贵阳医学院,贵州贵阳550004

出　　处：《中国医药工业杂志》2016年第2期163-167,共5页Chinese Journal of Pharmaceuticals

基　　金：贵阳市科技局现代药业计划项目[筑科合同(2011204)25号]给予资助

摘　　要：建立了生栀子、炒栀子、姜栀子及焦栀子的高效液相色谱指纹图谱,对比研究栀子炮制前后HPLC指纹图谱的变化。采用Diamosil C_(18)色谱柱,以甲醇∶0.1%磷酸梯度洗脱,检测波长238 nm(参照峰为栀子苷)、440 nm(参照峰为西红花苷I)。在238 nm指纹图谱中,栀子及各炮制品共标出19个特征峰。各炮制品的总峰面积值变化与生栀子的总峰面积值相比均无统计学意义(P>0.05)。在440 nm指纹图谱中,生栀子、炒栀子和姜栀子共标出15个特征峰,焦栀子标出17个特征峰。栀子生品及炮制品的指纹图谱中主要特征峰分布基本一致,但非共有峰分布呈显著差异。炒栀子、焦栀子的总峰面积与生栀子相比差异有统计学意义(P<0.05),西红花苷I含量显著下降。姜栀子总峰面积与生栀子比较差异无统计学意义(P>0.05)。The HPLC fingerprints of Fructu Gardeniaes, Fructus Gardeniae（stir-baked）, Fructus Gardeniae roasted with ginger juice and Fructus Gardeniae preparatus were established and compared to investigate the changes before and after processing. A Diamosil C_（18） column was used, with the mobile phase of methanol∶0.1% phosphate by gradient elution, at the detection wavelength of 238 nm and 440 nm, with the reference peaks of geniposide and crocin I, respectively. Nineteen common peaks of Fructus Gardeniae and its processed products were marked at 238 nm. The total peak area of each processed products and that of Fructus Gardeniae had no significant difference（P〉0.05）. Seventeen common peaks of Fructus Gardeniae preparatus and fifteen common peaks of Gardenia and other processed products were detected at 440 nm. Fingerprints of Fructus Gardeniae and its processed products in the overall drawing distribution of main characteristic peaks were basically same, but there were obvious differences for the distribution of non-common peaks. Compared with Fructus Gardeniae, the total area values and crocin I peak area value in fingerprint of Fructus Gardeniae（stir-baked） and Fructus Gardeniae preparatus decreased substantially（P〈0.05）. But the total area values in fingerprint of Fructus Gardeniae roasted with ginger juice had no obvious change（P〈0.05）.

关 键 词：栀子 炮制品 指纹图谱 高效液相色谱 

分 类 号：O657.72[理学—分析化学]