基于SSR的辽宁铁岭地区平榛遗传多样性与居群遗传结构分析  

An assessment of the genetic diversity and population genetic structure concerning the Corylus heterophylla Fisch.,grown in the Tieling district of Liaoning province,using SSR markers

作　　者：赵爽[1] 苏淑钗[1] 陈志钢[1] 盛淑艳 王忠[2] 

机构地区：[1]北京林业大学林学院.省部共建森林培育与保护重点实验室,北京100083 [2]辽宁省铁岭市林业科学研究院,辽宁铁岭112000

出　　处：《果树学报》2016年第1期24-33,共10页Journal of Fruit Science

基　　金：国家林业局重点项目"榛子良种选育与栽培关键技术研究"(2011-03)

摘　　要：【目的】研究我国铁岭地区平榛的遗传多样性,指导平榛优良品种选育工作。【方法】采用SSR分子标记的方法,对铁岭市3个平榛主产区共124株平榛材料进行遗传多样性和居群遗传结构分析。【结果】利用12株表型差异大的平榛植株,从56对SSR引物中筛选出12对条带清晰、多态性强的引物。12对引物在124株平榛材料中共扩增出75个基因位点,平均等位基因数为6.25个,观测杂合度(Ho)平均值为0.535 6,预期杂合度(He)的平均值为0.615 7。Shannon’s信息指数(I=1.274 2)和Nei’s多样性指数(H=0.613 2)较高,遗传分化系数(Fst=0.061 6)较低,基因流(Nm=7.599 3)较高,居群间遗传距离(GD<0.214 5)较小,遗传一致度(GI>0.806 9)较大。【结论】研究结果表明我国铁岭地区平榛的遗传多样性较高,居群间的遗传分化水平较低,且在大部分位点均表现偏离Hardy-Weinberg平衡,杂合度不足,居群间有较高的基因流。【Objective】The Corylus heterophylla Fisch. is widely found throughout the north of China,pri-marily within the three northeastern provinces. The total forest area of hazelnut is 1.67 million hm2 in ourcountry,and the proportion of the C. heterophylla is reaching 95% or more in our country. The Tielingwild hazelnut has a long history. To date,the area of the C. heterophylla in Tieling has reached up to80 000 hm2. Although the hazelnut has entered into the period of artificial cultivation,the higher qualityvarieties have still not been selected. Wang made a genetic diversity and genetic structure analysis of 14 C. heterophylla in our country using SSR molecular markers. This analysis provided a good foundation forthe application of SSR markers in the variety improvement of the C. heterophylla. However,her study hada large sampling range and low sampling quantity. The material she used did not involve the hazelnuts ofTieling,in which the C. heterophylla are widely distributed. In order to study the genetic diversity of the C. heterophylla in the Tieling area,and provide a guide for the breeding of hazelnuts,the genetic diversi-ty and population structure analysis of the C. heterophylla within three regions of Tieling were analyzed byusing SSR markers.【Methods】124 C. heterophylla from the three main-cultivation regions of Tieling were used as the materials for our study. These samples consist of 84 plants from Huangjingou,20 plantsfrom Xiaohongshi and 20 plants from Kaiyuan. SSR molecular markers were used for performing the genet-ic diversity and genetic structure analysis of the 124 C. heterophylla plants. The genome DNA was ob-tained using a new type of plant genomic DNA extraction kit. Then 1.0% agarose gel electrophoresis wasemployed for DNA integrity detection. We used an ultraviolet spectrophotometer to extract the purity andconcentration of the genomic DNA. The concentration of DNA was diluted to 10 mg · μL-1and it wasstored at-20 ℃ for later use. In order to screen the most suitable annealing tem

关 键 词：平榛 SSR 遗传多样性 遗传结构 

分 类 号：S664.4[农业科学—果树学]