‘襄汾圆枣’合子胚愈伤组织诱导成苗技术研究  

A regeneration system for zygotic embryo callus induction of Ziziphus jujuba‘Xiangfenyuanzao'

作　　者：杜学梅[1] 任海燕[1] 王玉国[2] 李登科[1] 赵爱玲 王永康[1] 薛晓芳[1] 隋串玲[1] 

机构地区：[1]山西省农业科学院果树研究所,果树种质创制与利用山西省重点实验室,山西太谷030815 [2]山西农业大学农学院,山西太谷030801

出　　处：《果树学报》2016年第2期233-240,共8页Journal of Fruit Science

基　　金：国家科技部科技支撑项目(2013BAD14B030101);山西省青年科技研究基金(2015021163);山西省留学办留学基金项目(2012-13);山西省农科院育种基础(Yyzjc1507);枣种质资源创新与利用山西省科技创新重点团队、果树种质创制与利用山西省重点实验室

摘　　要：【目的】获得胚性后代植株,优化枣合子胚培养条件,完善枣合子胚培养技术,从而为提高枣树新品种选育效率提供理论依据。【方法】以败育程度较轻的优良晚熟鲜食品种‘襄汾圆枣’枣盛花后30 d(球形胚)、40 d(心形胚)合子胚为外植体,进一步优化胚乳看护培养条件,并利用愈伤组织途径获得再生植株。【结果】适宜胚龄30 d、40 d的合子胚沿胚性生长的培养基分别为MS+IBA 0.2 mg·L-1+ZT 1.0 mg·L-1+GA35.0 mg·L-1+NAA 0.2 mg·L-1+蔗糖7%+LH 0.5 g·L-1、MS+IBA 0.5 mg·L-1+ZT 0.5 mg·L-1+GA35.0 mg·L-1+NAA 0.3 mg·L-1+蔗糖5%+LH 0.5 g·L-1,成胚率分别为54.17%、55.25%;不同胚龄的襄汾圆枣合子胚发育所需适宜的激素配比不同,在30 d胚龄阶段,NAA是影响成胚率的主要激素因子,而在40 d胚龄阶段,IBA则成为影响成胚率的主要激素因子。合子胚愈伤组织诱导的适宜培养基为MS+BA1.5mg·L-1+蔗糖4%,诱导率达70.59%;适宜合子胚分化培养基为MS+TDZ 0.8 mg·L-1+IAA 0.5 mg·L-1+蔗糖3%,分化率为58.90%。适宜生根培养基为1/2MS+IBA 1.0 mg·L-1+蔗糖2%,生根率达80%以上,移栽成活率超过85%。【结论】通过合子胚愈伤组织途径获得完整胚培苗,为枣育种技术开辟了新途径。[Objective] Chinese jujube is known for severe embryo abortion especially at globular or heart- shape stage; and the artificial zygotic embryo culture starting from pre-globular stage is confronted with low seedling rate. So far, thereis no data about the seedling regeneration from immature zygotic embryo callus of jujube. This study attempts to generate the offspring developed from embryos by optimizing the culture conditions including the composition of growth regulators and the factors for callus regeneration. It is expected to improve the techniques of zygotic embryo culture and to provide new data for enhancing the theoretic basis for efficient development of new jujube varieties. [Methods ] One genotype （Ziziphus jujube ＇Xiangfenyuanzao＇） with moderate embryo abortion were used in this study The naturally pollinated em- bryos sampling at 30 d （at globular stage） and 40 d （at heart-shaped stage） were used as explants for eal-

关 键 词：'襄汾圆枣’ 合子胚 胚性生长 愈伤组织诱导 再生植株 

分 类 号：S665.1[农业科学—果树学]