碳离子束辐照选育截短侧耳素菌原生质体  

Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation

作　　者：都雯玥 陆锡宏[1] 李雪虎[1] 周翔[1] 梁剑平[1,2] 辛志君[1] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州理工大学生命科学与工程学院,兰州730000

出　　处：《辐射研究与辐射工艺学报》2016年第1期39-44,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家高技术研究发展计划(863计划)(2011AA10A214);甘肃省陇药产业发展专项(Y361010SJ0)项目资助~~

摘　　要：使用12C6+离子束辐照Clitopilus pinsitus原生质体至不同吸收剂量,运用琼脂柱预筛和96孔板固体发酵选育截短侧耳素高产变异株。结果显示,最佳12C6+离子束吸收剂量为1.5 Gy,在该吸收剂量下正变异率为37.58%。选育出C.pin15I5E和C.pin15II6B两株正变异株,其产量较出发菌株分别提高16.17%和15.47%。表明重离子束辐照原生质体是行之有效的工业微生物诱变育种方法。Clitopilus pinsitus protoplast was irradiated by 12C6＋ ion beam at varied absorbed doses for mutagenesis, and high-yielding pleuromutilin mutant strains were screened using high-throughput method and solid fermentation. The results showed that the optimum 12C6＋ ion beam absorbed dose is 1.5 Gy, and under this condition the positive mutation rate is 37.58%. By agar column prescreening and 96-plate solid fermentation, the mutant strains, C.pinl515E and C.pin 15Ⅱ6B, were selected. The yields of the strain increased by 16.17% and 15.47%, respectively, compared with that of the original strain. The research suggests that applying heavy ion irradiation to protoplast is a possible method to breed mutant strains.

关 键 词：碳离子束 辐照 Clitopilus pinsitus原生质体 高产变异株 截短侧耳素 

分 类 号：Q691.5[生物学—生物物理学] TL99[核科学技术—核技术及应用]