血清铁生物剂量计的可行性研究  

Development of a new biodosimetry of serum iron

作　　者：谢丽华[1] 张晓红[1] 胡晓丹[1] 陈峰[1] 张泽群[1] 李群[1] 张海黔[1] 

机构地区：[1]南京航空航天大学,南京210016

出　　处：《辐射研究与辐射工艺学报》2016年第1期45-50,共6页Journal of Radiation Research and Radiation Processing

基　　金：国防基础科学研究项目(B2520133007);国家自然科学基金(11575086;31400721);江苏省自然科学基金(BK20131355);江苏高校优势学科建设工程项目资助~~

摘　　要：研究人体外周血血清经急性γ射线照射后血清铁的浓度变化,探讨其作为生物剂量计的可行性。人体血清经不同剂量γ射线辐照,采用酶联免疫吸附试验(Enzymes linked immunosorbent assay,ELISA)法定量检测血清铁的浓度,建立血清铁浓度与吸收剂量之间的剂量-效应关系。结果显示,血清铁浓度随吸收剂量升高,血清铁浓度与吸收剂量成线性平方关系:男性[y=30 676.5+2 383.5x–202.7x2(r=0.99)];女性[y=26 974.4+1 611.8x–141.3x2(r=0.98)]。4 Gy剂量时间-效应关系研究发现离体血清铁在2 d内维持稳定。利用建立的线性平方剂量-效应曲线估算的吸收剂量与双盲剂量(1、2、4 Gy)接近,估算值的95%置信区间较窄。研究结果提示血清铁具有重建人体吸收剂量的潜能。The aim is to study the serum iron changes in vitro serum of human peripheral blood irradiated by Co-60 γ-rays and explore its feasibility as a biodosimetry. To establish the dose-response relationship, the concentration of serum iron in γ-rays irradiated serum was detected using ELISA （Enzymes linked immunosorbent assay）. The results demonstrated that serum iron level increased with absorbed dose, and the dose-response curve of serum iron concentration followed the linear-quadratic model： male [y=30 676.5＋2 383.5x-202.7x2 （r=0.99）], female [y=26 974.4＋ 1 611.8x-141,3x2 （r=0.98）]. Time-response relationship of 4 Gy demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve, the absorbed dose was close to the double-blind dose （1, 2 and 4 Gy）, and the 95% confidence interval of the estimated value was narrow. Therefore, the serum iron has the potential to be a new biodosimetry to reconstruct human exposure dose.

关 键 词：生物剂量计 血清铁 Γ射线 

分 类 号：Q691[生物学—生物物理学] TL72[核科学技术—辐射防护及环境保护]