LPS协同PLA经JNK信号通路诱导NCI-H292细胞分泌IL-6、IL-8  

Release of IL-6 and IL-8 in NCI-H292 cells by the synergism of PLA and LPS through JNK signal pathway

作　　者：陈冰[1] 张玲玲[1] 刘玉文[1] 范晓云[1] 

机构地区：[1]安徽医科大学第一附属医院老年医学呼吸内科,合肥市230022

出　　处：《实用医学杂志》2016年第2期185-188,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金青年科学基金项目(编号:81100027);安徽省高校省级自然科学研究项目(编号:KJ2011A176);安徽医科大学第一附属医院博士启动基金项目;安徽人社厅第九批学术和技术带头人及后备人选学术科研活动资助项目

摘　　要：目的:研究多聚左旋精氨酸(PLA)和脂多糖(LPS)协同诱导气道上皮细胞NCI-H292分泌白介素-6(IL-6)和白介素-8(IL-8)的信号通路机制。方法:将NCI-H292细胞分为空白组、PLA组、LPS组、PLA+LPS组,采用Western Blot检测各组JNK的磷酸化水平,并比较SP-600125作用与否对JNK磷酸化和IL-6、IL-8表达量的影响。结果 :PLA、LPS诱导NCI-H292 JNK磷酸化水平增加(P<0.05),两者有协同作用(P<0.01);LPS协同PLA诱导NCI-H292分泌IL-6、IL-8(P<0.05);SP-600125抑制PLA、LPS、PLA+LPS诱导的JNK磷酸化(P<0.05)和IL-6、IL-8分泌(P<0.05)。结论:JNK信号通路参与LPS协同PLA诱导NCIH292分泌IL-6和IL-8的过程。Objective To study the mechanism of signaling pathway through which PLA（Poly-L-arginine）and（lipopolysaccharide, LPS） synergistically activate the release of（interleukin-6, IL-6） and（interleukin-8, IL-8）in NCI-H292 cells. Methods NCI-H292 cells were grouped into control group, PLA group, LPS group and PLA ＋LPS group and the phosphorylation of JNK in each group was measured by Western blot. The IL-6 and IL-8 were detected by（enzyme-linked immunosorbent assay, ELISA）. Results PLA and LPS increased the phosphorylation of JNK in NCI-H292 cells（P 〈0.05）, and there was a synergistic effect between PLA and LPS（P 〈0.01）. PLA and LPS synergistically induced the release of IL-6 and IL-8 in NCI-H292 cells（P 〈0.05）. JNK was significantly inhibited by SP-600125 which was a specific inhibitor of JNK signaling（P 〈0.05）, and the expression of IL-6 and IL-8 stimulated by PLA, LPS and PLA ＋ LPS decreased statistically by SP-600125（P 〈0.05）. Conclusion JNK signal pathway participates in the release of IL-6 and IL-8 that is stimulated by the synergism of PLA and LPS in NCI-H292 cells.

关 键 词：多聚左旋精氨酸 脂多糖 JNK信号通路 白介素-6 白介素-8 NCI-H292 

分 类 号：R562.25[医药卫生—呼吸系统]