生物功能化磁性纳米粒子的制备及对内皮祖细胞定向引导的体外研究  

Preparation of Biological Functional Magnetic Nanoparticles and Study on the Effect of Guiding Endothelial Progenitor Cells InVitro

作　　者：马宝龙[1,2] 闫维[2] 陈佳龙[3] 齐鹏凯[2] 李建辉[2] 黄楠[2] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]西南交通大学材料先进技术教育部重点实验室,成都610031 [3]安徽医科大学口腔医院,合肥230032

出　　处：《生物医学工程学杂志》2016年第1期136-143,共8页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(81271701;31300792)

摘　　要：采用化学共沉淀法制备由左旋多巴包裹的四氧化三铁磁性纳米粒子,合成磁性纳米粒子之后使用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺盐酸盐(EDC)活化左旋多巴表面的羧基官能团,与特定的核酸适配子上的氨基发生脱水缩合反应,构建具有核酸适配子与左旋多巴修饰的磁性纳米粒子。通过X射线衍射(XRD)、红外检测(FT-IR)、粒径分析(SEM)、磁性能测定(VSM)等检测方法对磁性纳米粒子制备的各个阶段进行检测,并将改性磁性纳米粒子与内皮祖细胞(EPCs)共混培养,评价磁性纳米粒子的细胞相容性以及磁性纳米粒子与EPCs短时间的结合效果,并在外加磁场的作用下评价改性磁性纳米粒子对EPCs的定向引导效果。结果表明,制备的改性后磁性纳米粒子有良好的磁响应性,并且在一定的浓度范围内有较好的细胞相容性,短时间对EPCs具有较好的结合作用,初步实现了在体外动态环境下对EPCs的定向引导。Coprecipitation method was used to prepare triiron tetroxide magnetic nanoparticles enclosed in L-DOPA,and then EDC was used to activate the carboxyl group of L-DOPA after the nanoparticles were synthesized.The carboxyl group of L-DOPA formed amide bond with specific amino on the aptamer by dehydration condensation reaction.The surfaces of magnetic nanoparticles were modified with aptamer and L-DOPA.X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FT-IR）,nanoparticle size analysis（SEM）,magnetic measurement（VSM）and other testing methods were used to detect the magnetic nanoparticles in different stages.The endothelial progenitor cells（EPCs）were cocultured with the surface modified magnetic nanoparticles to evaluate cell compatibility and the combination effect of nanoparticles on EPCs in a short period of time.Directional guide of the surface-modified magnetic nanoparticles to endothelial progenitor cells（EPCs）was evaluated under an applied magnetic field and simulated dynamic blood flow condition.The results showed that the prepared magnetic nanoparticles had good magnetic response,good cell compatibility within a certain range of the nanoparticle concentrations.The surface modified nanoparticles could combine with EPCs effectively in a short time,and those nanoparticles combined EPCs can be directionally guided on to a stent surface under the magnetic field in the dynamic flow environment.

关 键 词：四氧化三铁 左旋多巴 核酸适配子 化学共沉淀法 

分 类 号：O614.811[理学—无机化学]