慢性铅暴露对大鼠脑组织XRCC1 mRNA表达的影响及其与氧化应激的关系  

Effects of chronic lead exposure on expression of XRCC1 gene mRNA and its relationships with oxidative stress in brain tissues of rats

作　　者：李炜娟 任清风[1,3] 徐群英[1] 张中伟[1] 李伟[1] 冯建高[1] 任晓慧[1] 肖元梅[1] 

机构地区：[1]南昌大学公共卫生学院,330006 [2]南昌大学抚州医学院 [3]九江学院临床医学院.附属医院

出　　处：《天津医药》2016年第2期166-169,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81160342);江西省自然科学基金资助项目(20122BAB205047);江西省教育厅科技项目(GJJ11312)

摘　　要：目的观察饮水铅暴露对大鼠大脑皮质、小脑、海马组织中X线交错互补修复基因1(XRCC1)m RNA表达的影响及其与氧化应激的关系。方法 40只SD大鼠根据体质量按随机区组法分对照组和4个铅暴露组:最低剂量组、低剂量组、中剂量组、高剂量组,对照组自由饮用去离子水,4个铅暴露组分别饮用100、200、400、800 mg/L的醋酸铅溶液,连续染毒60 d后取大脑皮质、小脑和海马。RT-PCR技术检测脑组织XRCC1 mRNA的表达量,并测定脑组织铅、过氧化氢酶(CAT)、谷胱甘肽(GSH)和过氧化氢(H_2O_2)的含量。结果与对照组比较,铅暴露组大鼠大脑皮质、小脑和海马中XRCC1 mRNA表达量、脑铅的含量和H_2O_2水平均升高(P<0.05);而CAT、GSH含量基本低于对照组(P<0.05);相关性分析显示铅暴露组大鼠大脑皮质、小脑和海马中XRCC1 mRNA表达量与脑组织铅含量呈正相关(r分别为0.608、0.438、0.470,P<0.01),与CAT、GSH呈负相关(r分别为-0.343、-0.465、-0.423,-0.383、-0.489、-0.366,P<0.05),与H2O2呈正相关(r分别为0.455、0.517、0.342,P<0.05)。结论铅可通过诱导细胞氧化应激而影响XRCC1 mRNA的表达。Objective To observe the effects of lead exposure through drinking water on the expression of XRCC 1 mRNA in cerebral cortex, cerebellum and hippocampus of rats and its relationship with oxidative stress. Methods Forty SD rats were divided randomly into five groups： control group and four exposure groups （100 rag/L, 200 rag/L, 400 mg/L and 800 mg/L lead acetate for 60 days respectively）. The expression of XRCC1 mRNA in brain was detected by RT-PCR tec nique after separation of cerebral cortex, cerebellum, and hippocampus. At the same time, lead content in brain tissue and catalase （CAT）, glutathione （GSH） and hydrogen peroxide （H2O2）were also detected. Results Compared with control group, the expression of XRCC 1 mRNA, content of lead and H202 levels were significantly higher in cerebral cortex, cerebellum and hippocampus of exposure groups （P 〈 0.05）, whereas the contents of CAT and GSH were significantly lower （P 〈 0.05）. There was a positive correlation between lead level and the expression of XRCC 1 mRNA in cerebral cortex, cerebellum and hippo- campus of exposure groups （r=0.608, 0.438 and 0.470,P 〈 0.01）. There was a negative correlation between the lead level and CAT and GSH （r=-0.343, -0.465,-0.423, -0.383, -0.489 and -0.366, P 〈 0.05）. A positive relationship was found between the lead level and H202 （r=0.455, 0.517 and 0.342, P〈 0.05）. Conclusion Lead exposure can affect the expression of mRNA gene in XRCC 1 through inducing cell oxidative stress.

关 键 词：铅 X线交错互补修复基因1 氧化应激 过氧化氢酶 谷胱甘肽 过氧化氢 脑组织 

分 类 号：R994.4[医药卫生—毒理学]