P-JAK2/P-STAT3与突变型p53蛋白在宫颈病变中的表达及临床意义  

Expression and clinicopathological significance of P-JAK2,P-STAT3 and mutant p53 proteins in cervical lesions

作　　者：马慧[1] 尹利荣[1] 王芳[1] 李洪林[1] 单舒 

机构地区：[1]天津医科大学第二医院妇科,300211

出　　处：《天津医药》2016年第2期221-225,共5页Tianjin Medical Journal

摘　　要：目的探讨宫颈病变中磷酸化JAK激酶2(P-JAK2)、磷酸化信号转导与转录因子3(P-STAT3)及突变型p53蛋白的表达及其临床意义。方法选取我科收治的宫颈鳞癌(SCC组)57例、低级别上皮内瘤变(LSIL组)36例、高级别上皮内瘤变(HSIL组)30例和正常宫颈(NC组)30例为研究对象,检测各组高危型人乳头瘤病毒(HR-HPV)感染情况,行HE染色、免疫组化检测P-JAK2、P-STAT3及突变型p53蛋白表达情况。结果 (1)SCC组的HR-HPV感染率、P-JAK2阳性表达率高于HSIL组、LSIL组及NC组(P<0.05)。(2)SCC组的P-STAT3、突变型p53阳性表达率高于LSIL组、NC组(P<0.05),与HSIL组比较差异无统计学意义。(3)SCC组的P-JAK2和P-STAT3的阳性表达率在不同的FIGO分期、组织分化、淋巴结转移、HR-HPV感染亚组中有明显差异(均P<0.05);突变型p53的阳性表达率在不同的FIGO分期、HR-HPV感染亚组中具有显著差异(P<0.05)。(4)SCC组中P-JAK2与P-STAT3、突变型p53的阳性表达率、HR-HPV感染率均呈正相关(P<0.05),P-STAT3与突变型p53的表达率、HR-HPV感染率呈正相关(P<0.05);突变型p53表达与HR-HPV感染呈正相关(P<0.05)。结论 P-JAK2、P-STAT3与突变型p53蛋白在SCC组中的表达水平高于正常组及上皮内瘤变组,三者可能与HR-HPV感染、宫颈鳞癌发生、进展有关。Objective To investigate the expression and prognostic significance of P-JAK2, P-STAT3 and mutant p53 in cervical lesions. Methods A total of 153 cervical biopsies of patients from Gynecology Department, The Second Hospital of Tianjin Medical University were recruited during December 2013 to June 2015. Fifty-seven cases of squamous carcinoma of cervix （SCC）, 36 cases of low grade intraepithelial neoplasia （LSIL）, 30 patients with high grade intraepithelial neoplasia （HSIL） and 30 cases of normal cervix （NC） were included in the study. Gene chip method was used to detect high-risk hu- man papillpmavirus （HR-HPV）infection. Hematoxylin-eosin staining was used to make pathological diagnosis. Immunohistochemical assay was used for the detection of P-JAK2, P-STAT3 and mutant type p53 protein expression in cervical lesions. Results （1） HR-HPV infection rate and P-JAK2 expression were significantly higher in SCC group than those of HSIL group, LSIL group and NC group （P 〈 0.05）. （2） The expression of P-STAT3 and mutant type p53 were significantly higher in SCC group than those of LSIL group and NC group （P 〈 0.05）. However, there was no significant difference between SCC group and HSIL group. （3） The positive expressions of P-JAK2 and P-STAT3 showed significant differences in different FIGO stages, histopathological grade, lymph node metastasis and HR-HPV infection in SCC group, respectively （P 〈 0.05）. There were significant differences in the positive expression of mutant type p53 between different FIGO stages and HR-HPV infection （P 〈 0.05）. （4） There Was positive correlation between P-JAK2, P-STAT3, positive expression of mutant type p53 and HR-HPV infection in SCC tissues （P 〈 0.05）. There was a positive correlation between P-STAT3, p53 expression and HR-HPV infection （P 〈 0.05）. There was a positive correlation between mutant p53 expression and HR-HPV infection （P 〈 0.05）. Conclusion P-JAK2, P-STAT3 and mutant p53 protein expression rates

关 键 词：磷酸化JAK激酶2 磷酸化信号转导与转录因子3 突变型P53 宫颈鳞癌 高危型人乳头瘤病毒 宫颈上皮内瘤变 免疫组织化学 

分 类 号：R711.74[医药卫生—妇产科学]