基于腐蚀型光纤布喇格光栅的生物传感器  

Bio-sensor based on etched fiber Bragg grating

作　　者：周雨萌 赵春柳[1] 郎婷婷[1] 

机构地区：[1]中国计量学院光电子技术研究所,杭州310018

出　　处：《光通信技术》2016年第2期43-45,共3页Optical Communication Technology

基　　金：国家自然科学基金(61108058)资助;浙江省国际科技合作专项项目(2013C24018)资助

摘　　要：提出了一种将光纤传感技术与生物反应放大技术相结合的方案,实现了链酶亲和素浓度的检测。该方案采用硅烷偶联的方法对光纤进行表面改性,结合生物素-亲和素反应放大系统对生物浓度信号进行放大,并采用腐蚀型光纤布喇格光栅结构作为敏感元件将生物浓度信号转换为光信号进行表达。该传感器对目标物质具有特异性吸附能力,表明该传感器能准确识别链霉亲和素,在0mg/m L^1.0mg/m L浓度下灵敏度可达0.5452nm/mg/m L,能实现定量测量,在抗原抗体检测、蛋白质检测等领域有较好的应用前景。A streptavidin concentration sensor by the combination of the biotechnology and optical fiber sensing technology is proposed in this paper. The surfacecharacteristic of optical fiber is changed by silane coupling agents. A novel biotin-advin conjugation fiber probe is synthesized and employed in the detection of streptavidin. The fiber probe is made by etched fiber Bragg grating to be a sensitive part. The concentration of streptavidin can be monitored by the change of spectrum. The initial experiments demonstrate that the biotin have excellent absorbency to streptavidin, which means it is feasible to measure the concentration of streptavidin by this fiber sensor, showing a good application prospect in antigen and antibody detection or cancer cells examination.

关 键 词：链霉亲和素 光纤布喇格光栅 生物传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]