电针对慢性痛大鼠痛感觉和情绪成分相关杏仁核内μ-阿片受体等蛋白表达的影响  

Effect of Electroacupuncture Intervention on Expression of Pain Sensory and Affective Processing-relatedμ-opioid Receptor,etc.in the Amygdala in Chronic Neuropathy Pain Rats

作　　者：闫娅霞 冯秀梅[1] 王俊英[1] 端木程琳 陈淑萍[1] 高永辉[1] 韩焱晶[1] 王双昆[1] 张建梁[1] 刘俊岭[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2016年第1期3-10,共8页Acupuncture Research

基　　金：国家自然科学基金(No.81273830;30973796;81202762;81273828;81473779);国家重点基础研究发展计划"973"中医专项(No.2007CB 512505;2013CB 531904)

摘　　要：目的:观察电针对慢性神经痛负性情绪(NA)大鼠痛感觉和情绪成分相关杏仁核内μ-阿片受体(MOR)等蛋白表达变化的影响,探讨电针镇痛的作用机制。方法:雄性Wistar大鼠随机分为正常组、模型组、电针组、麻醉电针组,每组8只。结扎大鼠左侧坐骨神经结合足底反复电刺激造成慢性神经痛NA模型。电针双侧"足三里"-"阳陵泉",每日1次,共7d。测量大鼠双侧足底热痛阈(缩足反应潜伏期,PWL),计算其差值(PWLD)及在条件控制箱停留时间;采用免疫荧光、免疫印迹技术检测中央杏仁核及右侧杏仁核内MOR、环磷酸腺苷反应元件结合蛋白(p-CREB)、α-氨基羟甲基异噁唑丙酸受体亚单位GluR 1(GluA 1)、磷酸化细胞外信号调节激酶1和2(p-ERK 1/2)的表达。结果:与正常组比较,模型组PWLD显著增加(P<0.001),条件箱停留时间显著减少(P<0.001);电针7d后,电针组或麻醉电针组PWLD明显降低(P<0.05),电针组在条件箱停留时间显著增加(P<0.05),而麻醉电针组条件箱停留时间无明显变化(P>0.05),说明电针干预提高痛阈、减轻NA,麻醉电针组则抑制了电针改善NA的作用。此外,与正常组比较,模型组杏仁核MOR蛋白表达显著增加(P<0.05),GluA 1、p-ERK 2、p-CREB蛋白表达显著降低(均P<0.05)。电针7d后,电针组该4个蛋白,麻醉电针组MOR、p-ERK 2及p-CREB蛋白表达均显著上调(P<0.001,P<0.01,P<0.05);与电针组比,麻醉电针组GluA 1表达明显降低(P<0.05),而MOR、pERK 2、p-CREB的表达差异无统计学意义(P>0.05)。结论:重复电针可明显改善慢性痛大鼠痛感觉成分和情感成分,其改善痛情感成分的效应可能与上调大鼠杏仁核GluA 1蛋白表达相关,而杏仁核内MOR、ERK 2、CREB参与痛的感觉和情感成分的作用有待进一步探讨。Objective To observe the effect of electroacupuncture（EA）on expression of pain sensory and affective processing-relatedμ-opioid receptor（MOR）,glutamatergic AMPA receptor subunit GluA 1,extracellular signal-regulated kinase（ERK 1/2）,cAMP response element binding protein（CREB）in the amygdala in chronic constrictive injury（CCI）＋ negative affection（NA）rats,so as to reveal its mechanism underlying pain relief.Methods A total of 32 male Wistar rats were randomized into normal control,model,EA,and anesthesia＋EA（AEA）groups（n=8in each group）.The neuropathic pain NA model was established by ligation of the left sciatic nerve and repeated electrical stimulation of the paw-bottom in the pain-paired compartment.EA was applied to bilateral＂Zusanli＂（ST 36）and＂Yanglingquan＂（GB 34）for 30 min,once daily for 7days.Thermal pain threshold（paw withdrawl latency,PWL）of the bilateral paws was measured by using a Tail-Flick Unit.The expression levels of MOR and p-CREB in the central amygdala（CeA）and those of MOR,GluA 1,p-ERK 1/2and p-CREB in the right amygdala area were determined using immunofluorescence and Western blot,respectively.Results In comparison with the normal group,PWL difference（PWLD）values of the model group were significantly increased（P〈0.001）,and the time spent in the CPA-paired compartment was considerably decreased（P〈0.001）.After EA,the PWLD levels of both EA and AEA groups were apparently decreased（P〈0.05）,showing apain relief;and the time spent in the CPA-paired compartment was apparently increased in the EA group（P〈0.05）,rather than in the AEA group（P〉0.05）.Additionally,compared to the normal group,the expression level of MOR protein in the amygdala was remarkably increased（P〈0.05）and those of GluA 1,p-ERK 2and p-CREB proteins were apparently decreased（P〈0.05）.After EA intervention for 7days,the expression levels of these four proteins in the EA group,and those of MOR,p-ERK 2and p-CREB in the AEA group were si

关 键 词：电针 慢性痛 负性情绪 杏仁核 Μ-阿片受体 GluA 1 P-ERK 1/2 P-CREB 

分 类 号：R245.97[医药卫生—针灸推拿学]