电针对溃疡性结肠炎小鼠脾脏淋巴细胞Treg/Th17免疫平衡的影响  

Effect of Electroacupuncture Stimulation of “Guanyuan”(CV 4)and “Zusanli”(ST 36)on Spleen Lymphocytes Treg/Th 17 Immune Balance in Ulcerative Colitis Mice

作　　者：王程玉林 曾琳岚 耿煜[1] 王祥[1] 张何骄子 杨惠[1] 吴巧凤[1] 余曙光[1] 

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]资阳市疾病预防控制中心,资阳641300

出　　处：《针刺研究》2016年第1期55-59,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81373737s);教育部霍英东基础研究基金(No.141041);四川省科技厅青年基金项目(No.2015 JQO 058);四川省教育厅重点项目(No.15ZA 0103)

摘　　要：目的:观察电针对溃疡性结肠炎(UC)模型小鼠的治疗效应,并从调节性T细胞(Treg)与辅助性T细胞Th 17免疫平衡的角度探讨针灸治疗UC的机制。方法:健康雄性昆明种小鼠随机分为空白组、模型组、电针组,每组8只。采用3%葡聚糖硫酸钠法复制UC模型。电针组选取"关元""足三里"连续治疗5d,每天10min。观察各组小鼠体质量、大便黏稠度及出血情况,计算疾病活动指数;结肠组织HE染色,观察远端结肠组织病理形态学变化;流式细胞术检测小鼠脾淋巴细胞Treg、Th 17细胞水平。结果:电针对UC小鼠具有治疗效应,其疾病活动指数明显降低(P<0.05),结肠组织病理改变得到改善。与空白组相比,模型组CD4+CD25+Foxp3+Treg细胞水平下调,CD3+CD8+IL-17+Th 17细胞水平上调,差异具有统计学意义(P<0.05);电针治疗后,CD4+CD25+Foxp3+Treg细胞水平上调,CD3+CD8+IL-17+Th 17细胞水平下降,与模型组比较差异具有统计学意义(P<0.05)。结论:电针可能通过对UC小鼠Treg/Th 17免疫平衡的影响起到治疗UC的作用。Objective To observe the effect of electroacupuncture（EA）intervention on spleen T-helper 17（Th 17）and regulatory T（Treg）cell levels in mice with ulcerative colitis（UC）,so as to reveal its mechanisms underlying improvement of UC.Methods Kunming mice were randomized into control,UC model and EA groups,with 8mice in each group.The UC model was established by giving the mice with 3% Dextran Sulfate Sodium（DSS）for 5days.EA（15Hz/25 Hz,0.1-0.2mA）was applied at＂Guanyuan＂（CV 4）and bilateral＂Zusanli＂（ST 36,used alternatively）for 10 min,once a day for 5days.The animals’ disease activity index[DAI,=（body weight index score＋stool score＋bleeding score）/3;0-4points]were calculated.The pathological changes of colon tissues were observed by light microscopy after H.E.stain,and spleen Treg（CD4＋CD25＋Foxp3＋Treg）and Th 17（CD3＋CD8＋IL-17＋Th 17）lymphocyte levels were determined by flow cytometry.Results Compared to the control group,the DAI score and the ratio of Th 17/CD8＋T cells were significantly increased,while the ratio of Treg/CD4＋T cells obviously decreased in the model group（P〈0.05）.After EA intervention,the increased DAI score and the ratio of Th 17/CD8＋T cells and the decreased Treg/CD4＋T cells were reversed（P〈0.05）,and the inflammatory cell infiltration degree of the colon tissue was attenuated.Conclusion EA intervention can improve the UC rats’ symptoms of activity state,bloody or viscidity stool and colonic inflammation,probably by regulating the balance between the spleenic Treg and Th 17 lymphocytes.

关 键 词：溃疡性结肠炎 电针干预 脾 调节性T细胞 辅助性Th 17细胞 

分 类 号：R245.97[医药卫生—针灸推拿学]