隔药饼灸对功能性消化不良肝郁脾虚大鼠下丘脑单胺类神经递质表达的影响  

Influence of Herbal-cake-separated Moxibustion on Contents of 5-HT,DA and NE in Hypothalamus in Rats with Functional Dyspepsia of Liver Stagnation and Spleen Deficiency Syndrome

作　　者：肖靓宜 刘未艾[2] 吴清明[2] 王凌燕[1] 周魁明 叶海敏[2] 付磊[2] 

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410007 [2]湖南中医药大学第二附属医院针灸脑病科,长沙410005

出　　处：《针刺研究》2016年第1期60-64,共5页Acupuncture Research

基　　金：国家自然科学青年基金(No.81303049);湖南省中医药科研计划课题(No.2013117;2015133);国家自然科学基金资助项目(No.81173326);高等学校博士学科点专项科研基金资助项目(No.20124323110001)

摘　　要：目的：观察隔药饼灸对功能性消化不良（FD）肝郁脾虚型模型大鼠下丘脑组织中5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）的影响,探讨隔药饼灸治疗FD可能的作用机制。方法：SD大鼠按照随机数字表法随机分为空白组、模型组、隔药饼灸组、艾炷灸组、逍遥散组和多潘立酮组,每组10只。除空白组外其余5组采用复合病因造模法（慢性束缚应激＋过度疲劳＋饮食失节＋夹尾＋摇晃）造模,连续21d。隔药饼灸组、艾炷灸组施灸穴位均分为Ⅰ组（＂肝俞＂＂脾俞＂＂胃俞＂）和Ⅱ组（＂章门＂＂期门＂＂中脘＂）,两组穴位隔日交替,每穴连续灸4~5壮（约30min）;逍遥散组及多潘立酮组按1mL/100g分别灌服逍遥散水煎剂和多潘立酮水溶剂。分组治疗14d后,通过观察大鼠胃肠对营养性半固体糊的排空情况检测胃排空率,采用高效液相色谱-荧光法检测大鼠下丘脑组织中5-HT、DA、NE的含量。结果：与空白组比较,模型大鼠胃排空率明显下降（P〈0.01）,治疗结束后,各治疗组大鼠胃排空率较模型组上升（P〈0.01）,但各治疗组之间比较差异无统计学意义（P〉0.05）;与空白组比较,模型组大鼠下丘脑中的5-HT、DA、NE含量均显著下降（P〈0.01）,与模型组比较,隔药饼灸组、逍遥散组大鼠下丘脑组织中5-HT、DA、NE含量显著上升（P〈0.01）。结论：隔药饼灸、逍遥散均能显著增加FD肝郁脾虚模型大鼠下丘脑组织中的5-HT、DA、NE含量,从而调节下丘脑-垂体-肾上腺轴,提高大鼠胃排空率,达到改善胃肠动力的作用;艾炷灸、多潘立酮对FD肝郁脾虚模型大鼠下丘脑单胺类神经递质的作用不明显。Objective To observe the influence of herbal cake-separated moxibustion on the contents of 5-hydroxy tryptamine（5-HT）,dopamine（DA）and norepinephrine（NE）in the hypothalamus in rats with functional dyspepsia（FD）of syndrome of liver stagnation and spleen deficiency,so as to explore its mechanism underlying improvement of FD.Methods Sixty SD rats were randomly divided into 6groups：control,model,herbal cake-separated moxibustion,moxa-cone moxibustion,Xiaoyaosan（decoction for relieving liver stagnation）and Domperidon,with 10 rats in each group.The FD model was established by applying chronic restraint stress＋excessive fatigue＋irregular food＋tail clipping＋shaking for 21 consecutive days.Moxibustion（herbal cake-separated or moxa-cone）was applied to bilateral＂Ganshu＂（BL 18）,＂Pishu＂（BL 20）and＂Weishu＂（BL 21）,or＂Zhangmen＂（LR 13）,＂Qimen＂（LR 14）and＂Zhongwan＂（CV 12）for 30 min,once daily for 14 d.For rats of the two medication groups,Xiaoyaosan[1mL（2g）/100g]and Domperidone[1mL（0.3g）/100g]were administrated by gavage,respectively.The contents of 5-HT,DA and NE in the hypothalamus tissue were detected by high performance liquid chromatography.Results The gastric empty rate was obviously lower in the model group than in the control group（P〈0.01）.After the treatment,the gastric empty rate in the herbal cake-separated moxibustion,moxa-cone moxibustion,Xiaoyaosan and Domperidone groups were significantly increased in comparison with the model group（P〈0.01）,but there were no significant differences among the four treatment groups（P〉0.05）.Compared to the control group,the contents of 5-HT,DA and NE in the hypothalamus were markedly decreased in the model group（P〈0.01）,while compared to the model group,the contents of hypothalamic 5-HT,DA and NE contents were significantly up-regulated in both the herbal cake-separated moxibustion and Xiaoyaosan groups（P〈0.01）,rather than in the moxa-cone moxibustion and Domperidone gro

关 键 词：功能性消化不良 隔药饼灸 下丘脑 5-羟色胺 多巴胺 去甲肾上腺素 

分 类 号：R245.82[医药卫生—针灸推拿学]