β-丙内酯对流感病毒鸡胚高产母本株灭活条件的研究  

Study on the inactivation condition of β-propiolactone to influenza embryonated egg adapted high yield strains

作　　者：唐静[1] 鲁健[1] 郭俊峰[1] 舒跃龙[1] 辛丽[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《国际病毒学杂志》2016年第1期8-9,16,共3页International Journal of Virology

摘　　要：目的 流感病毒鸡胚高产母本株A/PR8/34株和X-157株灭活条件的研究.方法 应用改良后的β-丙内酯灭活法对流感病毒鸡胚高产母本株进行灭活.经试验证明,0.5‰β-丙内酯在37℃水浴,pH值范围在7.4-8.0之间条件下作用2h.补加0.5‰β-丙内酯,pH值为9,温度为4℃条件下过夜,能彻底灭活且保证流感病毒表面主要抗原的完整性.结果 采用改良后的β-丙内酯灭活法对流感病毒鸡胚高产母本株A/PR8/34株和X-157株灭活效果良好,鸡胚3代安检合格.灭活前后病毒血凝滴度不变.结论 改良后的β-丙内酯灭活法可用于流感病毒鸡胚高产母本株的灭活.Objective Study on the inactivation condition of β-propiolactone to influenza embryonated egg adapted high yield A/PR8/34 and X-157 strain.Methods Viruses was under 0.5‰ β-Propiolactone,37℃,PH value：7.4-8.0 condition for 2 hour.Then additional 0.5‰ β-Propiolactone was used and change PH value to 9,4℃ overnight.Results The reformed β-propiolactone inactivation method has a good effect on A/PR8/34 and X-157 strain.The HA titer was unchanged before and after activation.Conclusions The reformed β-Propiolactone Inactivation method can be used in the inactivate Influenza embryonated egg adapted high yield strains.

关 键 词：流感病毒 鸡胚高产母本株 β-丙内酯 灭活条件 

分 类 号：R373.32[医药卫生—病原生物学]