哺乳期过量的内源性大麻素干预导致成年鼠胰岛素抵抗  

作　　者：高啸[1] 许腾[1] 廖丹[1] 胡军[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院内分泌科,430077

出　　处：《浙江临床医学》2016年第3期425-427,共3页Zhejiang Clinical Medical Journal

摘　　要：目的评估长期使用内源性大麻素（AEA）对成年鼠的体重、附睾脂肪组织和代谢相关参数的影响。方法雄性鼠从哺乳期全程口服豆油乳化的AEA20μg/g。断奶后每10d对其摄食和体重进行记录。成年后行葡萄糖．胰岛素耐受测试。随后，处死小鼠得到其附睾脂肪，并对其血浆中的胰岛素、瘦素、非酯化脂肪酸、甘油三酯和胆固醇进行测定。结果哺乳期AEA处理组的小鼠在成年后表现出比较对照组较高的摄食量、较重的体重和较多的附睾脂肪。对附睾脂肪组织的CBR1蛋白的测定中，AEA处理组比对照组小鼠增高约1．5倍。AEA处理组小鼠外周循环中有较高的葡萄糖、胰岛素、瘦素、甘油三酯、胆固醇和非酯化脂肪酸。且AEA处理组小鼠表现出一系列显著的胰岛素抵抗的指标。结论在小鼠哺乳期使用AEA处理后其成年小鼠的体重、脂肪积累和对代谢产生干扰。Objective To evaluate long-term effects of AEA treatment during lactation on body weight, epididymal fat accumulation and related metabolic parameters during adulthood. Method Male mice pups were orally treated with a solution ofAEA （ 20 μ g/g body weight in soy oil ） or vehicle during the whole lactation period. After weaning, food intake and body weight were recorded every 10 days. Adult animals were subjected to glucose and insulin tolerance tests. Subsequently, animals were sacrificed and epididymal fat pads were extracted. Circulating levels of plasma insulin, leptin, non-sterified fatty acids （ NEFA ） , triglyceride and cholesterol were also evaluated. Results AEA-treated mice during lactation showed a significant increase in accumulated food intake, body weight and epididymal fat during adulthood when compared to control mice. When evaluating CB1R protein expression in epididymal fat, the AEA-treated group showed a 150 % increase in expression compared to the control mice. This group also displayed significantly higher levels of circulating glucose, insulin, leptin, triglycerides, cholesterol and NEFA. Moreover, a marked state of insulin resistance was an important finding in the AEA-treated group. Conclusion This study showed that overweight, accumulation of visceral fat and associated metabolic disturbances, such as a higher lipid profile and insulin resistance, can be programmed by a treatment with the endocannabinoid AEA during lactation in adult mice.

关 键 词：内源性大麻素 超重 胰岛素抵抗 内源性大麻素系统 脂肪组织 

分 类 号：R587.1[医药卫生—内分泌]