5个与脂肪酸代谢相关基因在油菜不同生育期表达量  

Expression of 5 fatty acid metabolism-related genes in different growth rapeseed stages

作　　者：刘凯[1] 肖刚[1] 张振乾[1] 官春云[1] 陈社员[1] 邬贤梦[1] 

机构地区：[1]湖南农业大学农学院,长沙410128

出　　处：《生物学杂志》2016年第1期32-34,48,共4页Journal of Biology

基　　金：国家自然科学基金(31201240;31000722);国家973计划(2015CB150206);863项目(2012AA101107-3);湖南省科技重大专项(2014FJ1006);农业部油料作物生物学与遗传育种重点实验室开放课题(2014008);湖南农业大学作物学开放基金(ZWKF201302)

摘　　要：为了明晰与脂肪酸代谢相关基因在不同油酸含量油菜中的表达情况,以出苗后7 d、5~6叶期和越冬期的叶片、花瓣和授粉后20~35 d的种子为材料,对5个转录组分析得到的差异基因（Bna0691280,Bna0421530,Bna0547440,Bna0799930和Bna0764010）在高油酸油菜近等基因系中的表达情况进行研究。结果发现,在营养生长期（出苗后7 d、5~6叶期和越冬期）的叶片中,5个基因在高油酸油菜材料中的表达量均高于低油酸油菜中的表达量。在花中除Bna0547440和Bna0764010外,其它3个基因在高油酸油菜中的表达量高于低油酸油菜中的表达量。在授粉后20~35 d的种子中除Bna0799930外,其余4个基因在高油酸油菜材料中的表达量均低于低油酸油菜中的表达量。研究结果可为高油酸油菜育种和分子机理研究提供参考。In order to understand the expression of fatty acid metabolism-related genes in rapeseed materials with different oleic acid contents,in this study,the leaves of 7-day seedlings,in 5- 6 leaf stage and overwintering period,petals and the seeds of 20- 35 days after pollination were used as materials, and the expression of 5 differential genes（ Bna0691280,Bna0421530,Bna0547440,Bna0799930 and Bna0764010） which obtained by transcriptome analysis of high oleic rapeseed near-isogenic lines were studied. The results showed that： in the leaves,the expression levels in high oleic rapeseed material were higher than those in the low oleic rapeseed. As for the expression in flowers,except for Bna0547440 and Bna0764010,the expression levels of the other 3 genes in high oleic rapeseed material were higher than those in low oleic rapeseed. While those in the seeds of 20- 35 days after pollination,the expression levels in the high-oleic rapeseed were lower than the expression in the low-oleic rapeseed except Bna0799930. The results of this study can be used for the breeding study of molecular mechanism of high oleic rapeseed.

关 键 词：实时荧光定量PCR 脂肪酸代谢 高油酸油菜 

分 类 号：S565.4[农业科学—作物学]