电刺激阴茎海绵体神经海绵体内压测定法在评价2型糖尿病大鼠模型勃起功能中的探讨  

Evaluation of the erectile function of Type 2 Diabetic rats through electrical stimulation of the cavernous nerve to monitor intracavernous pressure

作　　者：桂士良[1] 崔大伟[1] 腾飞[2] 曹会峰[1] 迟宝进[1] 罗振国[1] 陈锋[1] 杜丛林 郭玉刚[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154002 [2]吉林医药学院附属医院,吉林吉林132000

出　　处：《牡丹江医学院学报》2016年第1期16-19,共4页Journal of Mudanjiang Medical University

基　　金：黑龙江省教育厅科学技术研究项目(12521542)

摘　　要：目的探究电刺激阴茎海绵体神经测定海绵体内压(简称电刺激法)评价大鼠阴茎勃起功能在2型糖尿病大鼠模型中的应用。方法选清洁级雄性Wister大鼠40只,随机选择10只为对照组,标准饮食,余30只为实验组,予高脂高糖饮食,4周后予腹腔注射链脲佐菌素25mg/Kg/d,连续两天,筛选出10只2型糖尿病大鼠。两组均电刺激阴茎海绵体神经后测量海绵体内压(ICP)和平均颈动脉压(MAP),重复3次,每次间隔5min。结果糖尿病组大鼠3次测量ICP和MAP的结果无差异(P>0.05),正常组3次测量亦无差异(P>0.05),糖尿病组和正常组组间每次测量ICP和MAP的结果差异显著(P<0.05)。结论电刺激阴茎海绵体神经海绵体内压测定法在2型糖尿病大鼠模型中应用稳定,客观,灵敏,可反复,是评价2型糖尿病勃起功能障碍(ED)的理想方法。Objective To evaluate the erectile function in Type 2 Diabetic rats models through electrical stimulation of the cavernous nerve to measure intracavernous pressure（ ICP）. Methods Forty male clean grade Wister rats were randomized into 2 groups,10 rats were selected as control group with standard diet,and 30 rats were selected as experimental group. The experimental group were given high fat and sugar diet for 4 weeks,and were given Streptozotocin 25 mg / Kg / d through intraperitoneal injection for 2 days,then10 rats which were Type 2 Diabetic models were selected. The two groups rats were measured ICP and mean arterial pressure（ MAP）through electrical stimulation of the cavernous nerve for 3 times and 5 minutes at a time. Result The results of ICP and MAP from diabetic group had not significantly different between three replicate measurements（ P ﹥ 0. 05）. There was no significant difference among 3 times measurements in control group either（ P ﹥ 0. 05）. But the difference between diabetic group and control group were statistical significant（ P 〈0. 05）. Conclusion Electrical stimulation of the cavernous nerve to measure ICP of the Type 2 Diabetic models is stable,objective,sensitive and can be repeated,which is a ideal method to study Type 2 Diabetic erectile dysfunction（ ED）.

关 键 词：海绵体内压 平均动脉压 2型糖尿病 电刺激 勃起功能 海绵体神经 

分 类 号：R69[医药卫生—泌尿科学]