足三针对术后结肠胱硫醚γ裂解酶和髓过氧化物酶的影响  

Effects of Foot Three-needle Therapy on Colonic Cystathionine Gamma-lyase and Myeloperoxidase in Postoperative Rats

作　　者：赖名殷[1] 邓晶晶[2] 汪满霞 薛尔平 杨益宗 肖艳华[4] 陈津岩[5] 袁青[3] 

机构地区：[1]广州中医药大学科技产业园,广东广州510445 [2]广州市第八人民医院中医科,广东广州510440 [3]广州中医药大学针灸康复临床医学院,广东广州510405 [4]广州市第八人民医院病理科,广东广州510440 [5]广州中医药大学基础医学院,广东广州510405

出　　处：《广州中医药大学学报》2016年第1期46-50,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81202756);广东省自然科学基金资助项目(编号:S2012040007674)

摘　　要：【目的】观察针刺对结肠组织胱硫醚γ裂解酶(CSE)和髓过氧化物酶(MPO)的影响,探讨针刺促进结肠吻合术后胃肠起搏细胞Cajal间质细胞(ICCs)修复的机制。【方法】选用30只SD大鼠随机分为正常组、模型组(结肠吻合术)和针刺组。针刺组术后给予针刺双侧足三针(足三里、三阴交、太冲)。观察各组小肠推进率,采用免疫组织化学法检测术后大鼠结肠组织中酪氨酸激酶(c-kit)阳性ICCs的变化,以酶联免疫吸附(ELISA)法检测CSE的活性,生化法检测MPO的活性。【结果】与正常组比较,模型组小肠推进率显著下降,结肠组织c-kit阳性ICCs显著减少,结肠组织CSE和MPO活性显著升高(均P<0.05)。针刺组较模型组小肠推进率显著加快,结肠组织c-kit阳性ICCs显著增加,CSE和MPO活性显著降低(均P<0.05)。【结论】针刺能促进胃肠功能的恢复,其作用机制可能与调整术后结肠组织CSE和MPO活性以促进受损部位c-kit阳性ICCs修复有关。Objective To observe the effect of acupuncture on cystathionine gamma-lyase（CSE）and myeloperoxidase（MPO）in the colonic tissues of rats and to explore its mechanism for recovering the function of interstitial cells of Cajal（ICCs）in rats after colonic anastomosis. Methods Thirty SD rats were randomized into normal control group,model group（receiving colonic anastomosis）and acupuncture group. The acupuncture group received foot three-needle therapy on bilateral Zusanli（ST36）,Sanyinjiao（SP6） and Taichong（LR3）,once a day for 3 days after colonic anastomosis. And then the propulsive rate of the small intestine was measured. The count of ICCs with positively expressive c-kit in rat colonic tissues was measured by immunohistochemistry, the activity of CSE was observed by enzyme- linked immunosorbent assay（ELISA）and the activity of MPO was tested by biochemical method. Results Compared with the normal control group,the small intestinal propulsive rate in the model group was decreased, the number of ICCs with positively expressive c-kit was reduced, while the activities of CSE and MPO were increased（P〈0.05）. The acupuncture group had higher intestinal propulsive rate,more ICCs with positively expressive c-kit,and lower CSE and MPO activities than the model group（P〈0.05）.Conclusion Acupuncture can promote the recovering of postoperative gastrointestinal function, and its mechanism may be related to the regulation of CSE and MPO activities in the colonic tissues and to the restoration of ICCs function in the focus with positive c-kit.

关 键 词：术后胃肠动力障碍/针灸疗法 结肠/病理学 疾病模型 动物 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]