RP-HPLC测定小儿清热片中栀子苷和龙胆苦苷的含量  

Determination of Geniposide and Gentiopicroside in Xiao'er Qingre Tablets by RP-HPLC

作　　者：郭娜[1] 梁咏倩 宋粉云[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《食品与药品》2016年第1期41-44,共4页Food and Drug

基　　金：广东省自然科学基金资助项目(No.5002841)

摘　　要：目的建立小儿清热片中栀子苷和龙胆苦苷的含量测定方法。方法采用反相高效液相色谱法（RPHPLC）,Diamonsil C18柱,甲醇-0.1%磷酸水溶液（21：79）为流动相,流速1.0 m L/min,检测波长254 nm,柱温为室温。结果栀子苷在6.32~126.4μg/m L范围内线性关系良好（r=0.9998）,平均回收率为100.9%,方法精密度（RSD）为0.76%（n=6）;龙胆苦苷在2.04~40.8μg/m L范围内线性关系良好（r=0.9997）,平均回收率为100.1%,RSD为0.84%（n=6）。结论该法可用于小儿清热片中栀子苷和龙胆苦苷的含量测定。Objective To establish a method for determination of geniposide and gentiopicroside in Xiao＇ er Qingre Tablets. Methods A RP-HPLC method was established. The chromatographic column was Diamonsil C18. The mobile phase was a mixture of methanol-0.1% phosphoric acid （21：79, v/v）. The flow rate was 1.0 mL/min, and the detection wavelength was 254 nm. Results The linear range of geniposide was 6.32-126.4 μg/mL （t=0.9998）. The average recovery for geniposide was 100.6 %, with RSD of 0.76 % （n=6）. The linear range of gentiopicroside was 2.04-40.8 μg/mL （r=0.9997）. The average recovery for gentiopicroside was 100.1%, with RSD of 0.84 % （n=6）. Conclusion The method can be used for quantitative determination of the preparation.

关 键 词：小儿清热片 栀子苷 龙胆苦苷 反相高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]