藏药材牛尾蒿中总黄酮提取工艺优化及质量分数测定  

Optimization of the Extraction Technology for Total Flavonoids from the Tibetan Medicine Artemisia subdigitata Mattf.and Determination of Their Content

作　　者：杨正明[1] 滕云[1] 王景富[1] 李波[1] 刘圆[2] 

机构地区：[1]西南民族大学化学与环境保护工程学院,成都610041 [2]西南民族大学民族医药研究院,成都610041

出　　处：《西南大学学报（自然科学版）》2016年第2期176-181,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(81173653);西南民族大学研究生创新型科研项目(CX2015SZ061)

摘　　要：采用紫外分光光度法,以芦丁为对照品测定牛尾蒿中总黄酮的质量分数.通过单因素试验考察乙醇体积分数、提取时间、提取温度和料液比对总黄酮提取率的影响,并采用L9(34)正交试验优化藏药材牛尾蒿中总黄酮的提取工艺,测定各样品中总黄酮质量分数.结果表明:最佳提取工艺为乙醇体积分数20%,提取温度90℃,提取时间1.0h,料液比1∶50(g/mL),在此条件下,牛尾蒿中总黄酮提取率为67.88mg/g;不同植株部位总黄酮质量分数差异有统计学意义,以叶中质量分数最高;煅烧成炭后,总黄酮质量分数明显降低.优化后的提取工艺合理、可行,可用于牛尾蒿总黄酮的提取;牛尾蒿炭(闷煅)工艺的科学性和必要性有待进一步综合评价.Ultraviolet spectrophotometry was employed to determine the total flavonoids in Artemisia subdigitata Mattf.,with rutin as the control.The effects of ethanol concentration（v/v）,extraction time,extraction temperature and ratio of solid to liquid on the extraction rate of total flavonoids were evaluated in single-factor experiments,and an L9（34）orthogonal test was used to optimize the extraction technology.The results showed that the optimum extraction conditions were 20% ethanol,extraction temperature 90℃,extraction time 60 min,ratio of solid to liquid 1∶50（g/mL）,and the average extraction yield of total flavonoids was 67.88mg/g under the optimum conditions.The content of total flavoniods showed a large difference in different plant parts,being the highest in the leaves,and total flavoniods content decreased significantly after carbonization.This optimized extraction technology proved to be reasonable and feasible and is,therefore,recommended for use in the extraction of total flavonoids in A.subdigitata.Whether the process of carbonizing A.subdigitatais necessary and science-based is to be further evaluated.

关 键 词：牛尾蒿 牛尾蒿炭 总黄酮 正交试验 提取工艺 

分 类 号：R931[医药卫生—生药学]