天花粉凝集素对2型KK-Ay糖尿病小鼠血糖、血脂的调节作用  

Effects of Trichosanthes kirilowii Lectin on Blood Glucose and Serum Lipid in Type 2KK-Ay Diabetic Mice

作　　者：李琼[1] 张鹏[2] 郭晨[1] 牛光强 李学刚[1] 

机构地区：[1]西南大学药学院,重庆400715 [2]重庆市第九人民医院,重庆400700

出　　处：《西南大学学报（自然科学版）》2016年第2期182-188,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家科技部重大新药创制项目(2010ZX09401-306-3-10);重庆市科技攻关项目(2010AC5007);中央高校基本科研业务费(XDJK2015C159)

摘　　要：目的:探讨天花粉凝集素(Trichosanthes kirilowii lectin.TKL)对自发性2型KK-Ay糖尿病小鼠血糖、血脂的调节作用.方法:选取KK-Ay小鼠,随机分为模型组、阳性对照组(罗格列酮,0.003g/kg)、TKL(0.1,0.2,0.3g/kg)剂量组,以同龄C57BL/6J小鼠为正常组,连续灌胃30d,检测小鼠体质量、饮食量、饮水量、空腹血糖(FBG)、口服糖耐量、血清胰岛素(INS)及血脂等指标.结果:与模型组比较,各给药组体质量、饮食量、饮水量、FBG及INS值有显著下降(p<0.05,p<0.01),小鼠糖耐量异常也有所改善;30d后,TKL(0.3g/kg)组血清中TC、TG有明显改善(p<0.05),LDL-C明显降低,HDL-C明显升高(p<0.01);TKL(0.2g/kg)组血清中TG、LDL-C、HDL-C值有明显变化(p<0.05).结论:TKL对2型KK-Ay糖尿病小鼠有一定的降血糖、调血脂作用.Objective：To explore the hypolycemic and hypolipidemic effects of Trichosanthes kirilowii lectin（TKL）in type 2KK-Ay diabetic mice.Methods：The transgenic male KK-Ay mice were randomly divided into five groups,including a diabetic model group,a metformin group,a TKL low-dose（0.1g/kg）group,a medium-dose（0.2g/kg）group and a high-dose（0.3g/kg）group.Male C57BL/6Jmice of the same age were the control group.The mice were orally administered with TKL repeatedly for 30 days,and then their body weight,food and water intake,fasting blood glucose and serum insulin were measured,and oral glucose tolerance test was carried out to test the severity of diabetic.At the end of the experiment,the serum levels of blood lipid in each group were evaluated.Results：Compared with those of the diabetic group,the body weight,food and water intake,fasting blood glucose and serum level of insulin of the TKL-treated groups significantly decreased after 30days（p〈0.05,p〈0.01）and the impaired glucose tolerance in KK-Ay diabetic mice was improved.High-dose of TKL（0.3g/kg）significantly improved the serum levels of TC and TG（p〈0.05）,decreased LDL-C index and increased HDL-C index in type 2KKAy diabetic mice（p〈0.01）;and medium-dose of TKL（0.2g/kg）also significantly improved the TG,LDL-C and HDL-C levels of the serum（p〈0.05）.Conclusion：TKL can regulate blood glucose and the levels of hyperlipidemia in type 2KK-Ay diabetic mice.

关 键 词：天花粉凝集素 KK-Ay糖尿病小鼠 血糖 血脂 

分 类 号：R963[医药卫生—微生物与生化药学]