超级杂交水稻种子内生细菌群落结构及其多样性  

Diversity of endophytic bacterial communities in seeds of super hybrid rice( Oryza sativa L. )

作　　者：刘洋[1] 赵燃[1] 黎妮[2] 曹艳花[1] 张超 白飞荣 张欣[2] 袁隆平[2] 王伟平[2,3] 程池[1] 

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100015 [2]杂交水稻国家重点实验室(湖南杂交水稻研究中心),湖南长沙410125 [3]南方粮油作物协同创新中心,湖南长沙410128

出　　处：《食品与发酵工业》2016年第1期31-36,共6页Food and Fermentation Industries

基　　金：杂交水稻国家重点实验室开放课题基金(2014KF06);国家高技术研究发展计划(863计划)-强优势水稻杂交种的创制与应用(2011AA10A101);北京市科技新星计划项目(Z141105001814095)

摘　　要：通过采用构建16S r DNA克隆文库的非培养方法,对超级杂交水稻品种"深两优5814"及"Y两优900"种子内生细菌群落结构多样性进行研究。实验表明,"深两优5814"种子内生细菌含13个OUT,第一优势菌属为Pantoea,丰度为89.44%,第二优势菌和第三优势菌分别为Flavobacterium及Methylobacterium;"Y两优900"种子内生细菌含13个OUT,第一优势菌属为Pantoea,丰度为76.11%,第二优势菌和第三优势菌分别为Pseudomonas及Xanthomonas。由研究可见,泛菌属细菌为水稻种子中的优势菌群,同时,水稻种子基因型对其内生细菌的丰度及多样性具有一定的影响。The community diversities of endophytic bacteria in seeds of two types of super hybrid rice（ ＂Shenliangyou 5814＂and＂Y Liangyou 900＂） were studied through culture-independent method of the 16 S r DNA clone library technique. Experimental result showed that the endophytic bacterial communities in ＂5814＂included 13 OTUs,and the abundance of the first dominant bacterium Pantoea was 89. 44%,the second and third dominant bacterium was Flavobacteriu and Methylobacterium. The endophytic bacterial communities in ＂900 ＂included 13 OTUs,and the abundance of the first dominant bacterium Pantoea was 76. 11%,the second and third dominant bacterium was Pseudomonas and Xanthomonas. From this study,we found that Pantoea was the dominant genera in hybrid rice seeds,and the endophytic bacterial community structure had certain relevance to the seeds of hybrid rice which had been genetically related.

关 键 词：超级杂交水稻 种子内生细菌 非培养方法 群落多样性 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]