索骨丹化学成分研究  

Chemical Compositions of Rodgersia aesculifolia

作　　者：闫梦茹 马养民[1] 王健[1] 屈子睿[1] 许倩[1] 苗智[1] 

机构地区：[1]陕西科技大学化学与化工学院教育部轻化工助剂化学与技术重点实验室,西安710021

出　　处：《中国实验方剂学杂志》2016年第3期36-39,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家公益性行业(林业)科研专项(200904004)

摘　　要：目的:系统研究太白山地区索骨丹化学成分,为进一步研究其药理作用提供理论依据。方法:采用硅胶柱色谱、凝胶柱色谱、重结晶等方法分离纯化化合物,并结合化合物的理化性质和波谱分析鉴定其结构。通过最小抑菌浓度法对所得化合物进行活性测试。结果:从索骨丹中分离得到8个化合物,分别为β-谷甾醇(1),鬼灯檠素(2),儿茶素(3),对羟基苯甲酸(4),3β-hydroxyolean-12-en-27-oic acid(5),没食子酸(6),琥珀酸(7),苹果酸甲酯(8)。结论:化合物4,5,7和8均为首次从该植物中分离得到,其中化合物5是首次从该属植物中分离得到,而且化合物5对大肠杆菌有较好的抑菌活性,最小抑菌浓度为15.6 mg·L-1。Objective： To systematically study the chemical compositions of Rodgersia aesculifolia from Taibai Mountains,and provide theoretical basis for its further pharmacological research. Method： The compounds from R. aesculifolia were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization method. Their structures were identified according to their physicochemical properties and spectra analysis. A minimal inhibitory concentration（ MIC） method was used in antibacterial activity test for the compounds. Result： Eight compounds were isolated and identified as β-sitosterol（ 1）,bergenin（ 2）,catechin（ 3）,p-hydroxybenzoic acid（ 4）,3β-hydroxyolean-12-en-27-oic acid（ 5）,gallic acid（ 6）,succinic acid（ 7）,and methylmalic acid（ 8）. Conclusion： Compounds 4,5,7 and 8 were isolated from the plant of R.aesculifolia for the first time and compound 5 was first obtained from the genus. Compound 5 showed good inhibitory activities against Escherichia coli,with MIC value of 15. 6 mg·L- 1.

关 键 词：索骨丹 对羟基苯甲酸 3β-hydroxyolean-12-en-27-oic acid 琥珀酸 

分 类 号：R284.1[医药卫生—中药学]