HPLC同时测定川芎-天麻药对中4种指标性成分的含量  

Simultaneous Determination of Four Index Components in Extracts of Ligusticumc chuanxiong-Gastrodia elata by HPLC

作　　者：周赛妮 刘明平[1] 文艺[2] 严萍[3] 毛禹康 黄志云[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]广州中医药大学第一附属医院,广州510405 [3]广州中医药大学中药资源研究与工程中心中药资源科学教育部重点实验室,广州510006

出　　处：《中国实验方剂学杂志》2016年第3期53-56,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274107)

摘　　要：目的:建立川芎-天麻药对中天麻素、天麻苷元、阿魏酸和藁本内酯4种成分的含量测定方法。方法:采用Inertsil ODS-3色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.05%磷酸水梯度洗脱,检测波长220 nm(天麻素、天麻苷元),320 nm(阿魏酸、藁本内酯),柱温25℃,流速1.0 m L·min-1。结果:4种效应成分分离度良好,标准曲线在检测范围内线性关系良好,天麻素、天麻苷元、阿魏酸和藁本内酯的平均回收率分别为104.0%,103.4%,100.6%,101.8%,RSD分别为0.4%,0.5%,4.5%,2.6%。结论:该方法简便、精密度、重复性好,可用于川芎-天麻药对中4种效应成分的含量测定。Objective： To establish a method for the content determination of gastrodin, 4-hydroxybenzyi aicohol,ferulic acid and ligustilide in extracts of Ligusticumc chuanxiong-Gastrodia elata. Method：The determination was performed on Inertsil ODS-3 chromatographic column（ 4. 6 mm × 250 mm,5 μm） with mobile phase of methanol-0. 05% phosphoric acid for gradient elute,at the flow rate of 1. 0 m L·min- 1. The detection wavelength was set at 220 nm（ gastrodin,4-hydroxybenzyi aicohol）,320 nm（ ferulic acid,ligustilide）and column temperature was 25 ℃. Result： The four compounds were well separated and showed good linearity within the concentration ranges tested. Their average recovery rate was 104. 0%,103. 4%,100. 6% and 101. 8%for gastrodin,4-hydroxybenzyi aicohol,ferulic acid and ligustilide; and RSD of the above four components was0. 4%,0. 5%,4. 5% and 2. 6%,respectively. Conclusion： This method is simple,precise and repeatable,and it can be used to determine the four active components in Ligusticumc chuanxiong-Gastrodia elata.

关 键 词：天麻素 天麻苷元 阿魏酸 藁本内酯 川芎-天麻药对 

分 类 号：R284.1[医药卫生—中药学]