赶黄草的指纹药动学  

Fingerprint Pharmacokinetics of Aerial Part of Penthorum chinense

作　　者：陀扬凌 刘宇[1] 娄涛涛 欧小洪 张旭[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,成都611175

出　　处：《中国实验方剂学杂志》2016年第3期85-88,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省教育厅重点项目(2010A036)

摘　　要：目的：通过研究赶黄草的指纹药动学,为揭示中药血清指纹成分群吸收、分布和代谢规律提供参考。方法：大鼠灌胃给药,眼静脉丛采血,用甲醇沉淀蛋白,采用HPLC分析血清样品,流动相乙腈（A）-0.1%甲酸水（B）梯度洗脱（0~70min,10%~50%A;70~75 min,50%~10%A）,检测波长280 nm,柱温30℃,流速0.8 m L·min-1。建立赶黄草给药后血清样品的指纹图谱,确定入血成分。结果：发现6个入血成分,连续灌胃给药3 d后总入血成分浓度达到饱合,停药36 h后,基本代谢完全。结论：给药后血清中6个移行成分可能为赶黄草的药效物质基础;总入血成分药-时曲线变化规律与药物代谢动力学的吸收、分布、代谢过程类似,血清指纹成分群达到饱和时间较长,与中药起效慢、作用时间久的特点吻合,为中药的指纹药动学、药效物质基础及半衰期研究提供新思路。Objective： To reveal absorption,distribution and metabolic rule of serum fingerprint component group of traditional Chinese medicine by investigating fingerprint pharmacokinetic of Penthorum chinense. Method： Rats were administered orally, blood sampling by ophthalmic venous plexus, serum was separated by centrifugation. HPLC was employed to analyze serum samples with mobile phase of acetonitrile-0. 1%formic acid for gradient elution, flow rate of 0. 8 m L ·min- 1and detection wavelength at 280 nm. Based on establishment of serum fingerprints of P. chinense, total constituents absorbed into blood were determind by comparing fingerprints of ethanol extract, medicated serum sample and blank serum sample. Result： Six components were found into the blood including three prototype components and three metabolic components.Concentration-time curves revealed that total concentration had reached saturation after 3 days and removed all components after 36 h. Conclusion： Six components may be basis for efficacy substances in P. chinense,change law of total concentration-time curves is similar with absorption, distribution and metabolism process in pharmacokinetics. Components group of serum fingerprint reaches saturation with long time,which is the same as traditional Chinese medicine（ TCM） takes a slower effect and keep a long time. It can provide new ideas for fingerprint pharmacokinetics,pharmacodynamics material base and half-life study of TCM.

关 键 词：赶黄草 血清指纹图谱 指纹药动学 药-时曲线 灌胃给药 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]