痹祺胶囊水提取物及其单体成分抗炎活性比较  

Anti-inflammatory Activity Comparison of Aqueous Extract with Monomer Components of Biqi Capsule

作　　者：冯其帅 王贵芳[2] 王强松[1] 高丽娜[1] 崔元璐[1] 

机构地区：[1]天津中医药大学中医药研究院,天津300193 [2]天津中医药大学附属保康医院,天津300193

出　　处：《中国实验方剂学杂志》2016年第3期89-93,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2012ZX09304007)

摘　　要：目的:考察痹祺胶囊水提取物及其单体成分对脂多糖(LPS)诱导小鼠单核/巨噬细胞株RAW 264.7炎症反应的保护作用,以确定痹祺胶囊中抗炎的主要活性成分,为质量控制标准升级提供依据。方法:利用LPS诱导RAW 264.7细胞建立体外急性炎症模型,给予痹祺胶囊水提取物及6种单体成分马钱子碱,士的宁,隐丹参酮,丹酚酸B,甘草苷,甘草酸治疗,痹祺胶囊水提取物质量浓度分别为0.25,0.5,1 mg·L-1,各单体成分浓度分别为1×10-7,1×10-6,1×10-5mol·L-1,治疗24 h后,采用Griess试剂法和酶联免疫吸附测定法(ELISA)分别检测痹祺胶囊水提取物及各单体成分对LPS诱导RAW 264.7细胞分泌一氧化氮(NO)以及白细胞介素-6(IL-6)的影响。结果:与正常组比较,模型组RAW 264.7细胞NO,IL-6表达显著升高。与模型组比较,痹祺胶囊水提取物与隐丹参酮均能抑制NO和IL-6分泌;马钱子碱,士的宁,甘草苷和甘草酸均可抑制RAW 264.7细胞NO分泌。结论:痹褀胶囊水提取物中的主要抗炎成分是隐丹参酮,马钱子碱,士的宁。Objective： To observe the protective effect of aqueous extract and monomer components of Biqi capsule on lipopolysaccharides（ LPS）-induced inflammatory response of monocyte / macrophage cell line RAW 264. 7 in mice,determine the major anti-inflammatory active components of Biqi capsule,and provide theory basis for the upgrade of quality control standards. Method： Acute inflammatory model was built by LPS-induced RAW 264. 7 cells,and treated by aqueous extract of Biqi capsule（ mass concentration of 0. 25,0. 5,1 mg·L- 1）and major monomer components（ brucine, strychnine, cryptotanshinone, salvianolic acid B, liquiritin and glycyrrhizic acid,mass concentration of 1 × 10- 7,1 × 10- 6,1 × 10- 5mol·L- 1）. After treatment for 24 h,nitric oxide（ NO） and interleukin（ IL）-6 were detected by Griess reagent assay and enzyme-linked immunosorbent assay（ ELISA）,respectively. Result： Compared with the normal group,the expressions of IL-6 and NO were significantly increased in LPS-induced RAW 264. 7 cells of model group. Compared with the model group,both aqueous extract of Biqi capsule and cryptotanshinone could inhibit the increase of NO and IL-6; brucine,strychnine,glycyrrhizin and glycyrrhizic acid could inhibit NO secretion of RAW 264. 7 cells. Conclusion： Cryptotanshinone,brucine and strychnine are the major anti-inflammatory components in aqueous extract of Biqi capsule.

关 键 词：痹祺胶囊 水提取物 隐丹参酮 一氧化氮 白细胞介素-6 

分 类 号：R285.5[医药卫生—中药学]