四君子汤对脾虚大鼠空肠黏膜的修复及对葡萄糖吸收的影响  

Effects of Sijunzi Tang on Glucose Absorption and Jejunum Mucosa Repairing of Spleen Deficiency Rats

作　　者：黄丽花[1] 王素军[1] 钟运鸣[1] 熊晓红[1] 岑美凤 臧林泉[1] 王桂香[1] 

机构地区：[1]广东药学院药科学院,广州510006

出　　处：《中国实验方剂学杂志》2016年第3期94-97,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81302895);广东省中医药局基金项目(20132193)

摘　　要：目的:研究四君子汤对脾虚大鼠空肠黏膜损伤的修复作用及对葡萄糖吸收的影响,以探究四君子汤对脾虚证益气健脾的作用机制。方法:将SD大鼠分为正常组、模型组、精眯组(7.5 mg·kg-1)和四君子汤高、中、低剂量组(22.0,11.0,5.5 g·kg-1);除正常组外,ih利血平建立脾虚大鼠模型;通过大鼠在体单向肠灌流模型,运用紫外分光光度法测定不同组别大鼠肠道对葡萄糖的吸收,并使用光学显微镜对大鼠空肠组织的病理切片进行观察,比较其黏膜损伤修复情况。结果:通过对不同组别的葡萄糖吸收速率常数(Ka)和有效渗透系数(Papp)的比较显示,与正常组比较,模型组大鼠对葡萄糖的吸收显著降低,且空肠黏膜损伤明显(P<0.05);与模型组比较,精眯组和给药组大鼠对葡萄糖的吸收明显增加,空肠黏膜损伤明显得到修复(P<0.05)。结论:四君子汤能促进脾虚大鼠小肠吸收葡萄糖功能的恢复,其作用机制之一可能是通过修复脾虚大鼠的肠黏膜来增加小肠对葡萄糖的吸收,从而发挥益气健脾作用。Objective： To observe the effects of Sijunzi Tang on glucose absorption and the jejunum mucosa repair in spleen deficiency rats,and investigate the mechanism of Sijunzi Tang for replenishing Qi and invigorating the spleen. Method： The SD rats were divided randomly into 6 groups： normal group,model group,spermidine group（ 7. 5 mg·kg- 1） and Sijunzi Tang high dose group,middle dose group and low dose group（ 22. 0, 11. 0, 5. 5 g·kg- 1）. Spleen deficiency models were prepared by reserpine via subcutaneous administration in all rats except normal group. In situ single-pass intestinal perfusion models were used to determine rat intestinal absorption of glucose in different groups by ultraviolet spectroscopy. Light microscope was used to observe the pathological changes of jejunum tissues,and compare the repairing conditions of mucosal injury.Result： The glucose absorption rate constant（ Ka） and effective permeability coefficient（ Papp） in different groups showed that the glucose absorption was significantly lower in the model group than that in the normal group and the injury of jejunum mucosa was more obvious（ P〈0. 05）. Compared with the model group,the glucose absorption was significantly increased in rats of spermidine group and Sijunzi Tang groups,and the injury of jejunum mucosa was significantly repaired（ P〈0. 05）. Conclusion： Sijunzi Tang could improve the absorption of glucose via repairing the damaged structure of intestinal mucosa of spleen deficiency rats,which may be one of the possibly important mechanisms for replenishing Qi and invigorating the spleen.

关 键 词：四君子汤 脾虚证 葡萄糖 吸收 肠黏膜 

分 类 号：R285.5[医药卫生—中药学]