水稻HD9802S/Kasalath后代中高组织培养力家系的筛选  

Selection of Families with High Tissue Culture Ability in Filial Generation of HD9802S/Kasalath in Rice

作　　者：曾崇华 陈芬[1] 孟秋成[1] 于江辉[1] 肖友伦[1,3,4] 李锦江[1] 肖国樱[1] 

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,湖南长沙410125 [2]中国科学院大学,北京100049 [3]湖南农业大学生物科学技术学院,湖南长沙410128 [4]湖南省植物保护研究所,湖南长沙410125

出　　处：《杂交水稻》2016年第1期51-56,共6页Hybrid Rice

基　　金：转基因生物新品种培育科技重大专项(2014ZX08001-003)

摘　　要：HD9802S/Kasalath的杂交后代经多代农艺性状选择和自交纯合,共获得30个家系(291个选系)。对亲本、家系中高组织培养力相关的QTL(q Sv1和q Sc1-1)进行了检测,并对亲本、家系的成熟胚组织培养力进行了比较,结果发现亲本和家系均含有这2个QTL;HD9802S的愈伤组织诱导率低于Kasalath,绿苗分化率高于Kasalath,组织培养力与Kasalath相当;选出了9K18和9K19 2个组织培养力高的家系,其组织培养力分别为51.07%和54.80%,显著高于亲本HD9802S(26.88%)和Kasalath(27.12%),且其农艺性状明显优于Kasalath。Through pedigree selection of the cross of HD9802 S / Kasalath in target agronomic traits over generations,thirty filial families with 291 lines in total were acquired. The detection of QTLs（ q Sv1 and q Sc1-1） related to high tissue culture ability was conducted in 30 families and their parents,and the culture ability of mature embryo was compared among the parents and their filial families. The results showed that there existed both of the QTLs q Sv1 and q Sc1-1 in the parents and all families. HD9802 S had a lower callus induction rate but a higher green plantlet regeneration rate than Kasalath,so it had similar tissue culture ability to Kasalath. Two families of 9K18 and 9K19 with high tissue culture ability were selected for the culture ability of 9K18 and 9K19 was 51. 07% and 54. 80%,significantly higher than that of HD8902S（ 26. 88%） and Kasalath（ 27. 12%）,respectively. Besides,they were greatly improved in agronomic traits compared with the high culture ability parent of Kasalath.

关 键 词：水稻 愈伤组织诱导率 绿苗分化率 组织培养力 Kasalath 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]