碘普罗胺对大鼠肾小球脏层上皮细胞的影响  

Effect of iopromide on glomerular visceral epithelium cells

作　　者：于凤侠[1] 汪立杰[2] 林树无[1] 李子龙[3] 

机构地区：[1]沈阳医学院附属第二医院肾内科,辽宁沈阳110000 [2]中国医科大学第四附属医院心血管科,辽宁沈阳110000 [3]中国医科大学第一附属医院肾内科,辽宁沈阳110000

出　　处：《局解手术学杂志》2016年第2期83-86,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金项目(81170703)

摘　　要：目的探讨碘普罗胺诱导的大鼠对比剂肾病(CIN)发生的机制。方法 24只雌性SD大鼠随机分为正常对照组和对比剂肾病组(CIN组),各12只。CIN组采用尾静脉注射碘普罗胺的方法建立大鼠CIN模型;对照组尾静脉注射等量溶剂。所有大鼠均在注射后24 h处死。采用全自动生化分析仪测定24 h尿蛋白排泄量,免疫组化法测定肾小球上皮细胞细胞周期调控蛋白P21、P27、TGF-β1的阳性表达率,并用半定量评分法对各组大鼠的肾小球与肾小管间质的病理改变进行计量分析。应用TUNEL检测肾小球上皮细胞凋亡情况。结果与对照组相比,CIN组大鼠肾小球上皮细胞P21、P27、TGF-β1的阳性表达率显著增高,P21[(12.86±0.98)%vs(0.46±0.21)%,P=0.004 5],P27[(21.76±2.75)%vs(9.57±1.86)%,P=0.007 1]和TGF-β1[(12.85±5.54)%vs(7.63±0.84)%,P=0.003 7)];CIN组24 h尿蛋白显著性增加[(23.44±5.22)mg/d vs(2.13±0.52)mg/d,P=0.007 0];CIN组大鼠肾小球上皮细胞的病理损害更加严重和凋亡率明显增高[(52.5±6.4)%vs(4.2±0.3)%,P=0.007 5)],差异均有统计学意义。病理积分与24 h尿蛋白排泄量及P21,P27和TGF-β1的表达量呈正相关(r=0.765、0.701、0.842、0.651,P<0.01)。结论碘普罗胺可通过上调肾小球上皮细胞的P27、TGF-β1的表达及尿蛋白的排泄量,加重肾小球上皮细胞的病理损害及凋亡。Objective To investigate the mechanism of contrast-induced nephropathy（ CIN） caused by Iopromide. Methods Twofour female SD rats were randomly divided into two groups which were control group and CIN group. The rats in CIN group were injected Iopromide via caudal vein,the rats in control group were injected the equal amount of solvent. After 24 hours,all the rats were euthanized and tested. The excretion of 24 h urinary protein was detected using biochemistry assay. The expression of related cell cycle regulatory protein such as P21,P27 and TGF-β1 in glomerular visceral epithelium cells were measured using immunohistochemical technique. A semiquantitative score was used to evaluate the injure degree of glomerular and tubulointerstitium. Renal glomerular cell apoptosis was evaluated by TUNEL.Results Compared with control group,CIN group rat glomerular epithelial cells of P21,P27 and TGF beta 1 positive expression rate significantly increased,[（ 12. 86 ± 0. 98） % vs（ 0. 46 ± 0. 21） %,P = 0. 004 5 ],[（ 21. 76 ± 2. 75） % vs（ 9. 57 ± 1. 86） %,P = 0. 007 1 ],[（ 12. 85 ± 5. 54） vs（ 7. 63 ± 0. 84）,P = 0. 003 7） ] respectively,24 h urine protein significantly increase [（ 23. 44 ± 5. 22） mg/d vs（ 2. 13 ± 0. 52） mg/d,P = 0. 007 0],CIN pathological damage of rat glomerular epithelial cells and apoptotic rate significantly more serious[（ 52. 5 ± 6. 4） % vs（ 4. 2 ± 0. 3） %,P = 0. 007 5]. In addition,the renal pathologic scores were positively correlated with the excretion of24 hr urinary protein and the expression of P21,P27,and TGF-β1（ r = 0. 765,0. 701,0. 842,0. 651,P 〈 0. 01）. Conclusion Iodine amine via increased glomerular epithelial cells P27 and TGF-beta 1expression and urinary protein excretion,aggravating pathological damage and apoptosis.

关 键 词：对比剂肾病 碘普罗胺 肾小球上皮细胞 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学] R361.3[医药卫生—基础医学]