汇集浓缩血小板滤除白细胞前后质量检测分析  

The Quality Indicator Analysis of Pooled Platelet Concentrates after Removal of Leukocytes

作　　者：高加良[1] 雷宇[1] 万浬科[1] 王红[2] 袁理[2] 

机构地区：[1]四川省成都市血液中心,610041 [2]中国医学科学院,北京协和医学院,输血研究所

出　　处：《临床输血与检验》2016年第1期71-74,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的开展全血制备浓缩血小板的汇集及滤除白细胞的研究,为临床提供该血液品种提供质量保证的依据。方法采用富含血小板血浆法由400 ml新鲜全血制备浓缩血小板,并将6袋ABO同型的浓缩血小板汇集成1 U成人治疗量,用国产血小板型去白细胞滤器进行过滤,检测过滤前后血小板的相关质量指标。结果汇集血小板过滤前后的血小板数、红细胞混入量、白细胞混入量、p H值、低渗休克反应值分别为:(3.20±0.37)×10^(11)、(2.67±0.44)×10^(11)、(4.39±1.97)×10~9、(3.95±1.77)×10~9、(2.06±2.02)10~8、(0.02±0.03)×10~8、6.78±1.18、6.98±1.06、(68.53±9.18)%、(70.96±7.81)%;过滤后血小板回收率为:(82.67±5.58)%;过滤前后p H值、血小板低渗休克反应值的差异无统计学意义,其余指标的差异有统计学意义。结论将浓缩血小板进行汇集、过滤白细胞,可有效去除白细胞,质量指标符合相关标准要求,且过滤前后血小板功能无显著变化,值得临床推广应用。Objective To evaluate the quality of pooled platelet and leukocyte depletion, for supplying quality assurance for clinical transfusion. Methods Platelet concentrates （PCs） was prepared from 400 ml fresh whole blood by platelet rich plasma （PRP） method. 12 units of ABO-matched PCs were pooled, and filtered with the domestic filters. The indicators of PCs were tested before and after filtration. Results The number of platelet（Plt）, red blood celI（RBC）, leukocyte（WBC）, pH and hypotonic shock response（HSR） before and after filtration were （3.20±0.37）×10^11、（2.67±0.44）×10^11、（4.39±1.97）×10^9、（3.95±1.77）×10^9、（2.06±2.02）10^8、（0.02±0.03）×10^8、6.78±1.18、6.98±1.06、（68.53±9.18）%、（70.96±7.81）%. The platelet recovery was （82.67±5.58） %. There were significant difference（P 〈 0. 05） among these indicators, such as PH,RBC and WBC. Conclusion Pooling and filtering platelet concentrate is worthy of clinical use due to its standard quality and well-preserved function after filtration.

关 键 词：浓缩血小板 汇集 滤除白细胞 质量指标 

分 类 号：R331.14[医药卫生—人体生理学]