地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞Bax/Bcl-2表达的影响  

Effect of Rehmannia glutinosa oligosaccharides on Hydrogen peroxide-and serum deprivation-induced apoptosis in miniswine adipose tissue-derived stem cells

作　　者：裴志勇[1] 张健[1] 尹巧香[2] 杨桂利[3] 赵玉生[4] 

机构地区：[1]北京军区总医院干部病房一科,北京100700 [2]空军总医院干部病房心内科 [3]军事医学科学院基础医学研究所前沿交叉学科研究室 [4]解放军总医院老年心血管病研究所

出　　处：《中国临床保健杂志》2016年第1期69-71,113,共4页Chinese Journal of Clinical Healthcare

基　　金：国家高科技研究发展计划(2006AA02A105)

摘　　要：目的探讨地黄低聚糖(RGOs)对过氧化氢-血清饥饿诱导的小型猪脂肪组织来源干细胞(ASCs)Bax、Bcl-2表达的影响。方法过氧化氢(200μM)联合血清饥饿(H2O2/SD,6 h)建立小型猪ASCs凋亡模型。RGOs(0.01 g/L、0.1 g/L、1 g/L、10 g/L)预处理12 h并继续干预6 h。Western-blot法测定细胞Bax、Bcl-2的表达。结果 RGOs在一定浓度范围(0.1 g/L^10 g/L)可以减轻H2O2/SD引起的ASCs损伤,表现在Bax表达下调,Bcl-2表达上调。结论地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞的凋亡具有保护作用。Objective To investigate the effect of Rehmannia glutinosa oligosaccharides （RGOs） on hydrogen peroxide-and serum deprivation - induced apoptosis in adipose tissue-derived stem cells. Methods An apoptosis model in miniswine adipose tissue-derived stem cells （ASCs） was established by hydrogen peroxide-and serum deprivation-induced （H2 O2/SD ,6h）in vitro. RGOs （0.01 g/L,0.1 g/L, 1 g/L, 10g/L） pretreated for 12 hours and continued presence for 6 hours. Expression of Bax and Bcl-2 was investigated by using Western blot analysis. Results RGOs significantly attenuated hydrogen peroxide-and serum deprivation -induced ASCs apoptosis, showing a down -regulated Bax expres sion and an up-regulated Bcl-2 expression with the concentrations between O. lg/L - lOg/L. Conclution Rehmannia glutinosa oligosaccharides can protect adipose tissue-derived stem cells against hydrogen peroxide-and serum deprivationinduced apoptosis.

关 键 词：干细胞移植 地黄 细胞凋亡 过氧化氢 BCL-2相关X蛋白质 模型 动物 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]