不同活血中药配伍对脑缺血大鼠海马BDNF表达的实验研究  

Experimental Research on Effects of Different Compatibility of Blood Promoting Drugs on Expression of BDNF in Cerebral Ischemia Rats Hippocampus

作　　者：龙志江[1] 谢辉[1] 孟琼[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《中医药导报》2016年第2期20-23,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省科技厅研究项目(2012sk3142)

摘　　要：目的:观察补阳还五汤、血府逐瘀汤对局灶性脑缺血大鼠海马脑源性神经营养因子(BDNF)蛋白表达的影响,探讨不同活血配伍方法在不同时期对BDNF含量的影响。方法:将大鼠随机分为假手术组、模型组、补气活血组、行气活血组,共4组,每组30只,采用局灶性脑缺血再灌注损伤大鼠模型,造模后给予正常组、模型组生理盐水灌胃10 m L·kg^(-1),补气活血组、行气活血组分别灌胃中药26 g生药·kg^(-1),14mg生药·kg^(-1),1次/d,分别在第5、10、15天断头处死,每次每组大鼠10只。光镜下观察海马病理学变化,免疫组化检测BDNF蛋白的表达。结果:补气活血组、行气活血组BDNF含量明显升高,与模型组比较,差异有统计学意义(P<0.01);补气活血组在两周之内呈现缓慢持续不断增高趋势,行气活血组在第5天达到峰值,之后呈下降趋势;在第10、15天,补气活血明显高于行气活血组(P<0.01)。结论:补气活血和行气活血的配伍均能升高脑组织BDNF的含量,但是补气活血作用强于行气活血,对脑组织具有更好的保护作用。Objective： To observe the expression of brain-derived neurotrophic factor（BDNF） in rats hippocampus in focal cerebral ischemia after using Buyang Huanwu Decoction（BYHWD, 补阳还五汤） and Xuefu Zhuyu Decoction（XFZYD, 血府逐瘀汤）, to investigate the content of BDNF in different promoting blood circulation compatibility in different period. Methods： The rats were randomly divided into sham operation group,model group, Buqi Huoxue group, Xingqi Huoxue group, total of four groups, each 30 cases. Using focal cerebral ischemia-reperfusion injury in rat model, after modeling sham operation group and model group were given the normal saline 10 m L·kg^（-1）, Buqi Huoxue group and Xingqi Huoxue group were given liquid medicine 26 g·kg^（-1）,14mg·kg^（-1）, 1 times/d, the rats respectively were decapitated after 5,10,15 days, each 10 rats in each group. The pathological changes in hippocampus were observed under the light microscope, and the expression of BDNF was detected with immunohistochemical techniques. Results： BDNF was significantly higher in Buqi Huoxue group and Xingqi Huoxue group, there was a significant difference compared with the model group（P 〈0.01）.Buqi Huoxue group showed slow continuous increasing trend within two weeks, Xingqi Huoxue group showed downward trend after reaching a peak in the first five days. At day 10 th, 15 th, Buqi Huoxue group was significantly higher than Xingqi Huoxue group（P〈0.01）. Conclusions： Different promoting blood circulation compatibility of Buqi Huoxue and Xingqi Huoxue can increase BDNF levels in brain tissue, but the protection of Buqi Huoxue compatibility is stronger than the Xingqi Huoxue compatibility.

关 键 词：活血化瘀 补阳还五汤 血府逐瘀汤 脑缺血 脑源性神经细胞生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]